DOSE-RELATED EFFECTS OF ADENOSINE AND BRADYKININ ON MICROVASCULAR PERMSELECTIVITY TO MACROMOLECULES IN THE HAMSTER-CHEEK POUCH
DOSE-RELATED EFFECTS OF ADENOSINE AND BRADYKININ ON MICROVASCULAR PERMSELECTIVITY TO MACROMOLECULES IN THE HAMSTER-CHEEK POUCH
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DOI：
10.1161/01.res.58.3.348
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发表时间：
1986-03-01
影响因子：
20.1
通讯作者：
DURAN, WN
中科院分区：
文献类型：
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作者：
GAWLOWSKI, DM;DURAN, WN
仓鼠颊囊制剂用于评估微血管对三种血管舒张剂的选择性通透性反应：缓激肽、腺苷和罂粟碱。静脉注射异硫氰酸荧光素-葡聚糖150作为大分子示踪剂。为了量化渗透性的变化，我们根据 suffusate 与血浆异硫氰酸荧光素-葡聚糖 150 浓度的比率计算了荧光染料清除率值。使用落射荧光和明场光学显微镜将微循环记录在录像带上。局部应用缓激肽可引起大分子通透性的剂量依赖性增加。腺苷还以剂量依赖性方式增加渗透性。相对于对照，示踪剂清除率的增加对于 10-5 M 腺苷为 9.4 nl/min，对于 10-4 M 腺苷为 39.4 nl/min。这些剂量的标准误差为 1.5 nl/min。 10-6 M 腺苷不会改变通透性。 10-4M诱导的清除增量与缓激肽相当，为8倍。 10-7 M。用菲尼酮预处理对 10-5 M 腺苷介导的通透性反应没有影响。在一半的检查制剂中，局部应用罂粟碱增强了大分子的跨血管交换。同等剂量的腺苷的效果大约是其三倍。这项研究表明，腺苷与缓激肽一样，能够改变仓鼠颊囊中的微血管通透性反应。这种调节作用似乎是由于对毛细血管后微血管膜的直接作用。
The hamster cheek pouch preparation was used to assess microvascular permselectivity responses to three vasodilating agents: bradykinin, adenosine, and papaverine. Fluorescein isothiocyanate-dextran 150 was injected intravenously as a macromolecular tracer. To quantify changes in permeability, we calculated fluorochrome clearance values from the ratio of suffusate to plasma fluorescein isothiocyanate-dextran 150 concentration. The microcirculation was recorded on videotape, using epifluorescence and bright-field light microscopy. Topical application of bradykinin elicited dose-dependent increases in macromolecular permeability. Adenosine also augmented permeability in a dose-dependent fashion. The increases in tracer clearance, relative to control, were 9.4 nl/min for 10-5 M adenosine and 39.4 nl/min for 10-4 M adenosine. The standard error for these doses was 1.5 nl/min. Adenosine, 10-6 M, did not alter permeability. The increment in clearance induced by 10-4 M was comparable to that of bradykinin, 8 .times. 10-7 M. Pretreatment with phenidone had no effect on the permeability response mediated by 10-5 M adenosine. Topical application of papaverine enhanced the transvascular exchange of macromolecules in one-half of the preparations examined. Comparable doses of adenosine were approximately three times as effective. This study indicated that adenosine, like bradykinin, is capable of modifying microvascular permeability responses in the hamster cheek pouch. This modulatory effect appears to be due to a direct action on the postcapillary microvascular membrane.