Host cellular immune response to pneumococcal lung infection in mice
Host cellular immune response to pneumococcal lung infection in mice
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DOI：
10.1128/iai.68.2.492-501.2000
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发表时间：
2000-02-01
影响因子：
3.1
通讯作者：
Andrew, PW
中科院分区：
文献类型：
--
作者：
Kadioglu, A;Gingles, NA;Andrew, PW
虽然有大量证据表明肺炎球菌溶血素是肺炎球菌肺炎的重要毒力因子，但对其如何影响细胞浸润到肺中知之甚少。我们研究了突变型肺炎球菌不能产生肺炎球菌溶血素如何改变炎症和细胞浸润到肺部的模式。还评估了对肺中细菌生长的影响。野生型细菌在肺中的生长有三个阶段：下降，然后迅速增加，然后停滞或下降。肺炎球菌溶血素的缺乏与早期更快的下降，然后缓慢得多的增加数量。炎症细胞积累的模式也有不同的阶段，这些阶段的时间受到肺炎球菌溶血素的存在。中性粒细胞在感染野生型肺炎球菌后约12 ~ 16 h开始聚集，这种聚集发生在肺炎球菌数量早期下降之后，但与快速增长期一致。肺炎球菌感染后不能使肺炎链球菌溶血素，中性粒细胞流入较慢，强度较低。与肺炎球菌生长的第三阶段相一致的是T和B淋巴细胞在炎症部位的积累，但这种积累与肺中淋巴细胞总数的增加无关。在肺炎球菌溶血素的情况下发生淋巴细胞积累，但延迟。
Although there is substantial evidence that pneumolysin is an important virulence factor in pneumococcal pneumonia, relatively little is known about how it influences cellular infiltration into the lungs. We investigated how the inability of mutant pneumococci to produce pneumolysin altered the pattern of inflammation and cellular infiltration into the lungs. The effect on bacterial growth in the lungs also was assessed. There were three phases of growth of wild-type bacteria in the lungs: a decline followed by a rapid increase and then stasis or decline. The absence of pneumolysin was associated with a more rapid early decline and then a much slower increase in numbers. The pattern of inflammatory-cell accumulation also had distinct stages, and the timing of these stages was influenced by the presence of pneumolysin. Neutrophils began to accumulate about 12 to 16 h after infection with wild-type pneumococci, This accumulation occurred after the early decline in pneumococcal numbers but coincided with the period of rapid growth. Following infection with pneumococci unable to make pneumolysin, neutrophil influx was slower and less intense. Coincident with the third stage of pneumococcal growth was an accumulation of T and B lymphocytes at the sites of inflammation, but the accumulation was not associated with an increase in the total number of lymphocytes In the lungs. Lymphocyte accumulation in the absence of pneumolysin occurred but was delayed.