Antioxidant activities of chick embryo egg hydrolysates.
Antioxidant activities of chick embryo egg hydrolysates.
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鸡胚蛋水解物的抗氧化活性
DOI：
10.1002/fsn3.77
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发表时间：
2014-01
影响因子：
3.9
通讯作者：
Li, Bin
中科院分区：
文献类型：
--
作者：
Sun, Hao;Ye, Ting;Wang, Yuntao;Wang, Ling;Chen, Yijie;Li, Bin
鸡胚蛋水解物（CEEH）是通过体外模拟胃肠消化对鸡胚蛋进行酶水解而获得的。通过采用三种体外测定法研究了 CEEH 的抗氧化活性，包括 2,2'-azinobis-(3-乙基苯并噻唑啉-6-磺酸盐)/1,1-二苯基-2-三硝基苯肼 (ABTS/DPPH)/羟基自由基清除测定法。 CEEH（1.0mg/mL）的自由基清除作用呈剂量依赖性，其中ABTS、DPPH和羟基自由基的Trolox当量抗氧化能力最高，分别为569、2097和259.6μmol/L；而未孵化蛋对 ABTS、DPPH 和羟基自由基的 trolox 当量抗氧化能力分别为 199、993 和 226.5μmol/L。 CEEH对ABTS、DPPH和羟自由基等自由基的清除活性比未孵化蛋的水解产物更强。抗氧化氨基酸分析表明，14天的CEEH比未孵化的蛋具有更多的抗氧化氨基酸。此外，必需氨基酸分析显示，14天的CEEH营养价值最高。结合氨基酸谱的结果，CEEH 被认为比未孵化的鸡蛋除了抗氧化活性之外还具有更高的营养价值。
Chick embryo egg hydrolysates (CEEH) were obtained by enzymatic hydrolysis of chick embryo egg in vitro-simulated gastrointestinal digestion. The antioxidant activities of CEEH were investigated by employing three in vitro assays, including the 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonate)/1,1-diphenyl-2-picrylhydrazyl (ABTS/DPPH)/hydroxyl radical-scavenging assays. The radical-scavenging effect of CEEH (1.0 mg/mL) was in a dose-dependent manner, with the highest trolox equivalent antioxidant capacity for ABTS, DPPH, and that of hydroxyl radicals found to be 569, 2097, and 259.6 μmol/L, respectively; whereas the trolox equivalent antioxidant capacity of unhatched egg for ABTS, DPPH, and that of hydroxyl radicals were found to be 199, 993, and 226.5 μmol/L, respectively. CEEH showed stronger scavenging activity than the hydrolysates of unhatched egg against free radicals such as ABTS, DPPH, and hydroxyl radicals. The antioxidant amino acid analysis indicated that the 14-day CEEH possess more antioxidant amino acids than that of the unhatched egg. In addition, essential amino acids analysis showed that the 14-day CEEH have the highest nutritional value. Combined with the results of the amino acid profiles, CEEH were believed to have higher nutritive value in addition to antioxidant activities than the unhatched egg.