Characteristics and Precipitation of Glucose Oligomers in the Fresh Liquid Products Obtained from the Hydrolysis of Cellulose in Hot-Compressed Water
Characteristics and Precipitation of Glucose Oligomers in the Fresh Liquid Products Obtained from the Hydrolysis of Cellulose in Hot-Compressed Water
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DOI：
10.1021/ie900768m
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发表时间：
2009-09
影响因子：
4.2
通讯作者：
Yun Yu;Hongwei Wu
中科院分区：
文献类型：
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作者：
Yun Yu;Hongwei Wu
采用高效阴离子交换色谱-脉冲安培检测器（HPAEC-PAD），在280 °C和20 MPa的连续反应器系统中，对纤维素在热压水（HCW）中水解产生的新鲜液体产物中聚合度高达30的葡萄糖低聚物及其衍生物进行了研究。这些低聚物中没有一种可以通过具有蒸发光散射检测器的高效液相色谱法（HPLC-ELSD）检测到，然而，在液体溶液经由真空蒸发在40 °C下浓缩25倍之后，可以检测DP高达6的葡萄糖低聚物，在此期间形成大量沉淀。虽然由于缺乏标准品而无法对DP> 5的葡萄糖低聚物进行定量分析，但使用HPAEC-PAD和HPLC-ELSD对从葡萄糖（DP = 1）到纤维五糖（DP = 5）的葡萄糖低聚物的定量分析具有良好的一致性，这表明这些低DP葡萄糖低聚物具有良好的稳定性。
Using a high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), this study shows the presence of a wide range of glucose oligomers with the degrees of polymerization (DPs) up to 30 and their derivatives in the fresh liquid products produced from cellulose hydrolysis in hot-compressed water (HCW) using a semicontinuous reactor system at 280 °C and 20 MPa. None of those oligomers can be detected by a high-performance liquid chromatography with evaporative light scattering detector (HPLC-ELSD) that however can detect glucose oligomers with DPs up to 6 after the liquid solutions are concentrated by 25 times via vacuum evaporation at 40 °C, during which a large amount of precipitate is formed. While quantitative analysis of the glucose oligomers with DPs > 5 cannot be done due to the lack of standards, that of the glucose oligomers from glucose (DP = 1) to cellopentaose (DP = 5) using both HPAEC-PAD and HPLC-ELSD are in good agreement, suggesting that these low-DP glucose...