Cloning and analysis of a new developmentally regulated member of the basic helix-loop-helix family.
Cloning and analysis of a new developmentally regulated member of the basic helix-loop-helix family.
复制标题
基本螺旋-环-螺旋家族的一个新的发育调控成员的克隆和分析。
DOI：
10.1016/0925-4773(94)90019-1
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发表时间：
1994
影响因子：
2.6
通讯作者：
Maxwell,GD
中科院分区：
文献类型：
--
作者：
Helms,JA;Kuratani,S;Maxwell,GD
我们从胚胎鸡脑cDNA文库中分离出了碱性螺旋-环-螺旋（bHLH）家族成员。这3.86-Kb的cDNA，GbHLH1.4，表现出广泛的序列相似性的bHLH域与果蝇daughterless和脊椎动物的cDNA E12和HTF 4。在bHLH区域之外，相似性显著降低。GbHLH1.4识别4.0-Kb mRNA，原位杂交分析表明，GbHLH1.4 mRNA在发育的早期阶段广泛表达，但随着胚胎发生的进行而逐渐受到限制。在胚胎发育的后期阶段，mRNA转录物定位于几个结构，包括脊髓和大脑的心室层、面部原基、背根神经节和心肌以及心脏瓣膜。引人注目的是，GbHLH1.4在鸡胚中的表达表现出与鼠负HLH调节因子报道的显著重叠，同上。
We have isolated a basic helix-loop-helix (bHLH) family member from an embryonic chick-brain cDNA library. This 3.86-Kb cDNA, GbHLH1.4, exhibits extensive sequence similarity in the bHLH domain with Drosophila daughterless and the vertebrate cDNAs E12 and HTF4. Outside of the bHLH region the similarity is significantly reduced. GbHLH1.4 recognizes a 4.0-Kb mRNA and in situ hybridization analysis shows that GbHLH1.4 mRNA is widely expressed at early stages of development but becomes progressively restricted as embryogenesis proceeds. At later stages of embryonic development, mRNA transcripts are localized to several structures including the ventricular layers of the spinal cord and brain, the facial primordia, dorsal root ganglia and heart muscle and cardiac valves. Strikingly, GbHLH1.4 expression in chick embryos exhibits significant overlap with that reported for the murine negative HLH regulator, Id.