YUCCA Genes Are Expressed in Response to Leaf Adaxial-Abaxial Juxtaposition and Are Required for Leaf Margin Development
YUCCA Genes Are Expressed in Response to Leaf Adaxial-Abaxial Juxtaposition and Are Required for Leaf Margin Development
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YUCCA 基因响应叶近轴并置而表达，并且是叶缘发育所必需的
DOI：
10.1104/pp.111.186395
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发表时间：
2011-12-01
期刊：
影响因子：
7.4
通讯作者：
Xu, Lin
中科院分区：
文献类型：
--
作者：
Wang, Wei;Xu, Ben;Xu, Lin
在叶片发育过程中，原基期叶片近轴-远轴极性的形成对随后的叶片展开至关重要。然而，很少有人知道从极性建立叶片生长的遗传控制。叶缘，包括延长的边缘细胞和排水管，被认为是影响叶扩展。在这里，我们表明，突变体有缺陷的叶极性或功能丧失的多个生长素生物合成YUCCA（YUC）基因表现出类似的异常叶缘和少扩展的叶子。这些突变体的叶缘含有较少的包虫和细胞补丁的数量增加，其中表皮细胞的模式类似于包虫。以前的特点叶abaxialized不对称leping 2（as 2）revoluta（rev）和叶adaxialized kanadi 1（kan 1）kan 2双突变体都产生手指形，包虫样突起的近轴和远轴叶表面，分别。YUC是形成突起所必需的，因为as 2 rev和kan 1 kan 2产生的那些在yuc 1 yuc 2 yuc 4三重突变体背景中不存在。YUC 1，YUC 2和YUC 4的表达在叶中受到空间调节，与野生型叶中的积水点和as 2 rev和kan 1 kan 2叶上的突起相关。此外，抑制生长素运输的幼苗与N-（1-萘基）邻苯二甲酸或破坏生长素梯度转化植物的35 S：YUC 1构建体处理也阻止叶缘发育。总的来说，我们的数据表明，YUC在叶片中的表达对近轴-远轴并置做出反应，并且生长素的活性介导叶缘发育，从而促进叶片的生长。
During leaf development, the formation of leaf adaxial-abaxial polarity at the primordium stage is crucial for subsequent leaf expansion. However, little is known about the genetic control from polarity establishment to blade outgrowth. The leaf margin, comprising elongated margin cells and hydathodes, is thought to affect leaf expansion. Here, we show that mutants with defective leaf polarity or with loss of function in the multiple auxin-biosynthetic YUCCA (YUC) genes exhibited a similar abnormal leaf margin and less-expanded leaves. Leaf margins of these mutants contained fewer hydathodes and an increased number of cell patches in which the patterns of epidermal cells resembled those of hydathodes. The previously characterized leaf-abaxialized asymmetric leaves2 (as2) revoluta (rev) and leaf-adaxialized kanadi1 (kan1) kan2 double mutants both produce finger-shaped, hydathode-like protrusions on adaxial and abaxial leaf surfaces, respectively. YUCs are required for formation of the protrusions, as those produced by as2 rev and kan1 kan2 were absent in the yuc1 yuc2 yuc4 triple mutant background. Expressions of YUC1, YUC2, and YUC4 were spatially regulated in the leaf, being associated with hydathodes in wild-type leaves and protrusions on as2 rev and kan1 kan2 leaves. In addition, inhibition of auxin transport by treatment of seedlings with N-(1-naphtyl) phtalamic acid or disruption of the auxin gradient by transforming plants with the 35S:YUC1 construct also blocked leaf margin development. Collectively, our data show that expressions of YUCs in the leaf respond to the adaxial-abaxial juxtaposition, and that the activities of auxin mediate leaf margin development, which subsequently promotes blade outgrowth.