The rat cytochrome c oxidase subunit IV gene family: tissue-specific and hormonal differences in subunit IV and cytochrome c mRNA expression.
The rat cytochrome c oxidase subunit IV gene family: tissue-specific and hormonal differences in subunit IV and cytochrome c mRNA expression.
复制标题
大鼠细胞色素 c 氧化酶亚基 IV 基因家族：亚基 IV 和细胞色素 c mRNA 表达的组织特异性和激素差异。
DOI：
10.1093/nar/18.22.6581
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发表时间：
1990
影响因子：
14.9
通讯作者：
Scarpulla,RC
中科院分区：
文献类型：
--
作者：
Virbasius,JV;Scarpulla,RC
我们已经分离出大鼠细胞色素氧化酶亚基IV基因家族的三个成员：一个功能基因和两个加工假基因。假基因似乎代表了这个家族中唯一的其他密切相关的序列。该功能基因编码一种在所有检测的组织中表达的同种型，并具有“管家”基因的特征。这些包括多个转录起始位点映射到一个约50 bp的区域和一个富含GC的启动子缺乏典型的CCAAT或TATAA序列。虽然亚基IV基因在心肌和骨骼肌中表达水平最高，与这些组织中的高能量需求一致，但其表达在几个方面与细胞色素蛋白不同。1)与细胞色素mRNA的高度可变水平相比，不同组织中亚基IV mRNA的丰度相对均匀。2)与细胞色素不同，亚基IV mRNA在睾丸中以令人惊讶的高水平表达。3)虽然在肝脏中的cytochromecmRNA水平显着增加甲状腺激素治疗的反应，亚基IV mRNA没有显着影响。这两个核编码的呼吸基因的表达差异与其启动子内调控元件的差异一致。因此，核编码的呼吸基因在响应组织对细胞能量的需求时的调节可能不能用所有这些基因共有的一组通用调节因子来令人满意地解释。
We have isolated three members of the rat cytochromecoxidase subunit IV gene family: one functional gene and two processed pseudogenes. The pseudogenes appear to represent the only other closely related sequences in this family. The functional gene encodes an isoform which is expressed in all tissues examined and has features characteristic of ‘housekeeping’ genes. These include multiple transcription start sites mapped to within an approximately 50 bp region and a GC-rich promoter lacking typical CCAAT or TATAA sequences. Although the subunit IV gene is expressed at its highest levels in cardiac and skeletal muscle, consistent with the high energy demand in those tissues, its expression differs from that of cytochromecin several respects. 1) Subunit IV mRNA abundance in various tissues is relatively uniform when compared to the highly variable levels of cytochromecmRNAs. 2) Unlike cytochromec, subunit IV mRNA is expressed at a surprisingly high level in testis. 3) While cytochromecmRNA levels in liver are increased markedly in response to thyroid hormone treatment, subunit IV mRNA is not significantly affected. Differences in the expression of these two nuclear-encoded respiratory genes are consistent with differences in regulatory elements within their promoters. Therefore, the regulation of nuclear-encoded respiratory genes in response to tissue demands for cellular energy may not be satisfactorily explained by a set of universal regulators common to all such genes.