Joining mutants of RAG1 and RAG2 that demonstrate impaired interactions with the coding-end DNA
Joining mutants of RAG1 and RAG2 that demonstrate impaired interactions with the coding-end DNA
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DOI：
10.1074/jbc.m405485200
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发表时间：
2004-09-10
影响因子：
4.8
通讯作者：
Sakano, H
中科院分区：
文献类型：
--
作者：
Nagawa, F;Hirose, S;Sakano, H
在抗原受体基因的V（D）J连接中，两个重组信号序列（RSSs），12-和23-RSSs，与重组激活基因RAG 1和RAG 2的蛋白产物形成复合物。DNaseI足迹法表明RAG蛋白与底物RSS DNA的相互作用不仅限于信号区，还涉及编码序列。RAG 1和RAG 2的连接突变体表现出与前切割型复合物和后切割型复合物中的编码区的相互作用受损。在V（D）J重组的背景下讨论了这种RAG编码区相互作用的可能作用。
In V(D)J joining of antigen receptor genes, two recombination signal sequences (RSSs), 12- and 23-RSSs, form a complex with the protein products of recombination activating genes, RAG1 and RAG2. DNaseI footprinting demonstrates that the interaction of RAG proteins with substrate RSS DNA is not just limited to the signal region but involves the coding sequence as well. Joining mutants of RAG1 and RAG2 demonstrate impaired interactions with the coding region in both pre- and post-cleavage type complexes. A possible role of this RAG coding region interaction is discussed in the context of V(D)J recombination.