Methodology for detection and typing of foodborne microorganisms
Methodology for detection and typing of foodborne microorganisms
复制标题
DOI：
10.1016/s0168-1605(99)00081-1
复制
发表时间：
1999-09-15
影响因子：
5.4
通讯作者：
Beumer, RR
中科院分区：
文献类型：
--
作者：
de Boer, E;Beumer, RR
在过去的十年里，检测食品中致病细菌的传统和现代方法都有了许多改进。食品微生物学传统方法的改进和自动化包括样品制备、电镀技术、计数和鉴定检测试剂盒。ATP生物发光技术越来越多地被用于测量清洁表面和器皿的效率。细胞计数方法，包括流式细胞术和直接荧光滤膜技术，是适合快速检测微生物的技术，特别是在液体中。基于阻抗测量的自动化系统能够在一天内根据细菌总数筛选大量样本。多种形式的免疫分析使许多病原体的快速检测成为可能。最近，基于核酸的检测方法在食源性病原体的检测和分型方面有了重要的发展。通过使用聚合酶链式反应和其他扩增技术，这些方法的灵敏度显著提高。替代和快速方法必须满足在准确性、有效性、速度、自动化、样本矩阵等方面的几个要求。在危害分析关键控制点计划中使用常规方法和快速方法。可以期待进一步的改进，特别是在免疫分析和遗传方法方面，包括使用生物传感器和DNA芯片技术。(C)1999 Elsevier Science B.V.保留所有权利。
Over the past decade many improvements have been seen in both conventional and modern methods for the detection of pathogenic bacteria in foods. Modifications and automation of conventional methods in food microbiology include sample preparation, plating techniques, counting and identification test kits. ATP bioluminescence techniques are increasingly used for measuring the efficiency of cleaning surfaces and utensils. Cell counting methods, including flow cytometry and the direct epifluorescent filter technique are suitable techniques for rapid detection of microorganisms, especially in fluids. Automated systems based on impedimetry are able to screen high numbers of samples based on total bacterial counts within I day. Immunoassays in a wide range of formats make rapid detection of many pathogens possible. Recently, there have been important developments in the use of nucleic acid-based assays for the detection and subtyping of foodborne pathogens. The sensitivity of these methods has been significantly increased by the use of the polymerase chain reaction and other amplification techniques. Alternative and rapid methods must meet several requirements concerning accuracy, validation, speed, automation, sample matrix, etc. Both conventional and rapid methods are used within hazard analysis critical control point programs. Further improvements especially in immunoassays and genetic methods can be expected, including the use of biosensors and DNA chip technology. (C) 1999 Elsevier Science B.V. All rights reserved.