Aquaculture of the tropical sea cucumber, Stichopus monotuberculatus: Induced spawning, detailed records of gonadal and embryonic development, and improvements in larval breeding by digestive enzyme supply in diet
Aquaculture of the tropical sea cucumber, Stichopus monotuberculatus: Induced spawning, detailed records of gonadal and embryonic development, and improvements in larval breeding by digestive enzyme supply in diet
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热带海参（Stichopus monotuberculatus）的水产养殖：诱导产卵、性腺和胚胎发育的详细记录以及通过饲料中消化酶的供应来改善幼体繁殖
DOI：
10.1016/j.aquaculture.2021.736690
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发表时间：
2021-03-30
期刊：
影响因子：
4.5
通讯作者：
Chen, Ting
中科院分区：
文献类型：
--
作者：
Cheng, Chuhang;Wu, Feifei;Chen, Ting
单节刺参是最具价值的热带海参之一，具有很高的食用、药用和生态价值。本研究采用一种改良的低温刺激法诱导单瘤螺旋藻的产卵。详细描述了性腺的成熟、胚胎、仔鱼和幼鱼的发育，并通过添加多种消化酶的饲料促进了仔鱼的繁殖。结果表明，7月-9月是海南单节对虾中国的最适繁殖期。低温刺激比传统的干燥刺激更适合作为一种人工催产方法，因为它的死亡率低，受精卵发育成幼虫需要大约21d。与对照组相比，添加2 mg/Lα-淀粉酶、2 mg/L纤维素酶和5亩L/L海藻糖酶组6日龄存活率分别提高21.37±-0.054%、22.57±-0.088%和29.83±0.105%，10日龄存活率分别提高24.2±-0.094%、30.02±-0.152%和36.44±-0.052%。在饲料中添加1 mg/Lα-淀粉酶、1 mg/L纤维素酶和5亩L/L海藻糖酶，分别比对照组提高5.3+/-0.521倍、2.86/-0.521倍和4.66+/-0.656倍。本研究为单节对虾的规模化人工繁殖和可持续人工养殖提供了一种先进的方法。
Stichopus monotuberculatus is one of the most valuable tropical sea cucumbers, with high edible, medical and ecological values. In this study, the spawning of S. monotuberculatus was induced by a modified low-temperature stimulation method. Gonadal maturation, and embryonic, larval and juvenile development were described in detail, and larval breeding was improved by diets supplemented with multiple digestive enzymes. JulySeptember resulted as the optimal breeding period of S. monotuberculatus in Hainan Province, China. Lowtemperature stimulation was more suitable than traditional dry stimulation as a method for artificially inducing S. monotuberculatus spawning since its low mortality, whose fertilized eggs took approximately 21 days to develop into juveniles. Compared to the control group, the diets with 2 mg/L alpha-amylase, 2 mg/L cellulase and 5 mu L/L trehalase added increased the larval survival rates at 6 days by 21.37 +/- 0.054%, 22.57 +/- 0.088% and 29.83 +/- 0.105%, respectively, and at 10 days by 24.2 +/- 0.094%, 30.02 +/- 0.152% and 36.44 +/- 0.052%, respectively. For the larval settlement rate, supplement of 1 mg/L alpha-amylase, 1 mg/L cellulase and 5 mu L/L trehalase in the diet could cause 5.30 +/- 0.521-fold, 2.86 +/- 0.521-fold and 4.66 +/- 0.656-fold improvements relative to the control group, respectively. This study may contribute an advanced method for the large-scale artificial breeding and sustainable artificial culture of S. monotuberculatus.