Non-coding RNA LINC00473 mediates decidualization of human endometrial stromal cells in response to cAMP signaling.
Non-coding RNA LINC00473 mediates decidualization of human endometrial stromal cells in response to cAMP signaling.
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非编码 RNA LINC00473 响应 cAMP 信号传导，介导人子宫内膜基质细胞的蜕膜化。
DOI：
10.1038/srep22744
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发表时间：
2016-03-07
影响因子：
4.6
通讯作者：
Yang ZM
中科院分区：
文献类型：
--
作者：
Liang XH;Deng WB;Liu YF;Liang YX;Fan ZM;Gu XW;Liu JL;Sha AG;Diao HL;Yang ZM
蜕膜化是怀孕的一个重要步骤。然而，长的基因间非编码RNA（LincRNA）的功能的贡献，蜕膜化尚未探讨。为探讨LincRNA在人子宫内膜蜕膜化过程中的调控作用，采用雌二醇（E2）、环磷酸腺苷（db-cAMP）和醋酸甲羟孕酮（medroxy孕酮acetate）体外诱导人子宫内膜间质细胞（HESCs）发生蜕膜化。LINC 00473（LINC 473）表达在蜕膜刺激后在HESC中被高度诱导。我们发现cAMP-PKA通路通过IL-11介导的STAT 3磷酸化调节LINC 473的表达。RNA干扰介导的LINC 473下调抑制体外蜕膜化这些结果表明LINC 473可能是人类蜕膜化所必需的功能。这是第一份证明LincRNA在人类蜕膜化过程中存在的报告。
Decidualization is an essential step in the establishment of pregnancy. However, the functional contributions of long intergenic noncoding RNAs (LincRNAs) to decidualization have not been explored. To explore the regulation and role of LincRNAs during human decidualization, human endometrial stromal cells (HESCs) are induced to undergo in vitro decidualization by treating with estradiol-17β, db-cAMP and medroxyprogesterone acetate. LINC00473 (LINC473) expression is highly induced in HESCs after decidual stimulus. We found that cAMP-PKA pathway regulates the expression of LINC473 through IL-11-mediated STAT3 phosphorylation. RNA interference-mediated down-regulation of LINC473 inhibits in vitro decidualization. These results suggested that LINC473 might be functionally required for human decidualization. This is the first report demonstrating the presence of LincRNA during human decidualization.