Experimental Brain Ischemia: Neuron‐Specific Enolase Level in Cerebrospinal Fluid as an Index of Neuronal Damage
Experimental Brain Ischemia: Neuron‐Specific Enolase Level in Cerebrospinal Fluid as an Index of Neuronal Damage
复制标题
实验性脑缺血：脑脊液中神经元特异性烯醇化酶水平作为神经元损伤的指标
作者：
Steinberg;Gueniau;H. Scarna;Keller;TM. Worcel;F. Pujol
神经元特异性烯醇化酶（NSE）水平在阻断通往大脑的4条大动脉10、20或30分钟后测定。在全缺血30min的大鼠脑脊液中，NSE水平在最初几个小时内升高高达9倍，然后缓慢下降。显著水平可持续8天。缺血3天后的组织学观察显示，海马、纹状体和丘脑等前脑区域出现神经元丢失和新区域损伤。缺血后，探索行为和神经状态短暂下降，24小时后不再可见。缺血10或20 min后，NSE水平升高程度较轻，损伤神经元较少。缺血时间与脑脊液中NSE的释放量呈正相关，表明脑脊液中NSE的测定是神经元病变的敏感、可靠的指标。
Abstract: Levels of neuron‐specific enolase (NSE) were measured in rat CSF following occlusion of the four major arteries to the brain for 10, 20, or 30 min. In the CSF of rats submitted to 30 min of total ischemia, an up to ninefold increase of NSE level occurred within the first few hours and then slowly diminished. Significant levels were seen for as long as 8 days. Histological observations 3 days after ischemia showed neuronal loss as well as neu‐ ronal damage in several forebrain regions such as hippocampus, striatum, and thalamus. Ischemia was followed by transient decreases in exploration behavior and neurological states that were no longer visible 24 h later. After 10 or 20 min ischemia, NSE levels were increased to a lesser degree and fewer damaged neurons were observed. The positive correlation between duration of ischemia and amount of NSE release in CSF indicates that the measurement of NSE in the CSF is a sensitive and reliable index of neuronal lesions.