A motile Chlamydomonas flagellar mutant that lacks outer dynein arms.
A motile Chlamydomonas flagellar mutant that lacks outer dynein arms.
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一个缺乏外动臂的运动衣原体鞭毛突变体。
DOI：
10.1083/jcb.100.4.1228
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发表时间：
1985-04
影响因子：
7.8
通讯作者：
Rosenbaum, J L
中科院分区：
文献类型：
--
作者：
Mitchell, D R;Rosenbaum, J L
选择了一种新的衣原体鞭毛突变体，pf-28，它比野生型细胞游得更慢。薄切片电子显微镜显示完全没有外排动力蛋白武器在这个突变体，而内排武器和其他轴丝结构出现正常。SDS PAGE分析还表明，以前确定为外臂动力蛋白成分的多肽是完全不存在的PF- 28。在含有0.6 M KCl的蔗糖梯度上，该突变体保留的两种ATP酶在17.7S和12.7S处沉积。pf-28的总体游泳模式与野生型几乎没有区别，除了向前游泳速度降低到野生型值的35%，并且细胞在避恐期间几乎没有或没有向后运动。突变细胞会对趋光刺激做出反应，它们的鞭毛会以正向或反向模式跳动。这是第一次报告的突变，缺乏动力蛋白臂，可以游泳。
A new Chlamydomonas flagellar mutant, pf-28, which swims more slowly than wild-type cells, was selected. Thin-section electron microscopy revealed the complete absence of outer-row dynein arms in this mutant, whereas inner-row arms and other axonemal structures appeared normal. SDS PAGE analysis also indicated that polypeptides previously identified as outer-arm dynein components are completely absent in pf- 28. The two ATPases retained by this mutant sediment at 17.7S and 12.7S on sucrose gradients that contain 0.6 M KCl. Overall swimming patterns of pf-28 differ little from wild-type except that forward swimming speed is reduced to 35% of the wild-type value, and cells show little or no backward movement during photophobic avoidance. Mutant cells will respond to phototactic stimuli, and their flagella will beat in either the forward or reverse mode. This is the first report of a mutant that lacks dynein arms that can swim.