Activation markers of human basophils: CD69 expression is strongly and preferentially induced by 163
Activation markers of human basophils: CD69 expression is strongly and preferentially induced by 163
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DOI：
10.1067/mai.2002.123532
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发表时间：
2002-05-01
影响因子：
14.2
通讯作者：
Hirai, K
中科院分区：
文献类型：
--
作者：
Yoshimura, C;Yamaguchi, M;Hirai, K
背景：嗜碱性粒细胞的生物学功能在体外受到多种细胞因子的精确调控，但在细胞因子介导的活化过程中上调的表面标志物却知之甚少。目的：CD69、CD44和CD54是细胞因子介导的嗜酸性粒细胞活化的“激活标志物”。本研究的目的是阐明这些分子在体外和体内嗜碱性粒细胞中的表达和调节。方法：采用密度梯度离心法和负选择法从静脉血中纯化嗜碱性粒细胞。用流式细胞仪分析细胞表面的表达。同时检测哮喘患者支气管肺泡灌洗液和血液标本中嗜碱性粒细胞表面CD69、CD44和CD54的表达。结果：CD44和CD54在嗜碱性粒细胞上呈结构性表达，并被IL-3中度上调。另一方面，CD69仅在新鲜分离的嗜碱性粒细胞中有微弱的表达，而IL-3诱导了极高水平的表达。与CD63和CD11b相比，CD69出现的时间较慢，诱导表达在24小时内完成。嗜碱性细胞CD69没有功能相关性，但它确实有生物学相关性。哮喘患者的全血嗜碱性粒细胞表达CD69的水平明显高于正常人。此外，支气管肺泡灌洗液中嗜碱性粒细胞的CD69表达水平高于同一供者的外周血嗜碱性粒细胞。结论：IL-3优先且强烈上调嗜碱性粒细胞上CD69的表达。嗜碱性粒细胞表面CD69在体外和体内均可作为IL-3激活这些细胞的标志物。
Background: The biological functions of basophils are precisely regulated by various cytokines in vitro, but little is known about surface markers that are upregulated during the cytokine-mediated activation process.Objective: It has been well established that CD69, CD44, and CD54 represent "activation markers" for cytokine-mediated eosinophil activation. The objective of this study was to elucidate the expression and regulation of these molecules in human basophils in vitro as well as in vivo.Methods: Basophils were purified from venous blood by means of density gradient centrifugation followed by negative selection. Surface expression was analyzed by means of flow cytometry. We also studied the expression of CD69, CD44, and CD54 on basophils in bronchoalveolar lavage fluid and blood specimens from patients with asthma.Results: CD44 and CD54 were constitutively expressed on basophils and moderately upregulated by IL-3. On the other hand, CD69 expression was only weakly observed in freshly isolated basophils, but IL-3 induced extremely high levels of expression. Surface CD69 appeared rather slowly in comparison with CD63 and CD11b, and the induction of expression was completed within 24 hours. Basophil CD69 had no functional relevance, but it did have biological relevance. Whole blood basophils from asthmatic individuals expressed significantly higher levels of CD69 than did those from normal individuals. Furthermore, bronchoalveolar lavage fluid basophils showed higher levels of CD69 expression than did blood basophils from the same donors.Conclusion: CD69 expression on basophils was preferentially and strongly upregulated by IL-3. CD69 on basophils might be useful as an in vitro as well as in vivo marker of activation of these cells by IL-3.