RAT NEURAL ANTIGEN-2 (RAN-2) - A CELL-SURFACE ANTIGEN ON ASTROCYTES, EPENDYMAL CELLS, MULLER CELLS AND LEPTO-MENINGES DEFINED BY A MONOCLONAL-ANTIBODY
RAT NEURAL ANTIGEN-2 (RAN-2) - A CELL-SURFACE ANTIGEN ON ASTROCYTES, EPENDYMAL CELLS, MULLER CELLS AND LEPTO-MENINGES DEFINED BY A MONOCLONAL-ANTIBODY
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DOI：
10.1016/0006-8993(81)90593-x
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发表时间：
1981-01-01
期刊：
影响因子：
2.9
通讯作者：
WILLIAMS, CA
中科院分区：
文献类型：
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作者：
BARTLETT, PF;NOBLE, MD;WILLIAMS, CA
用培养的大鼠星形胶质细胞的富集群体免疫小鼠;将它们的脾细胞与[小鼠] NS-1骨髓瘤细胞融合以产生分泌抗体的杂交瘤。分离出两个稳定的杂交瘤克隆，其分泌单克隆IgG 2抗体，所述抗体与培养物中的绝大多数大鼠星形胶质细胞的表面反应。在间接免疫荧光试验中研究了其中一种抗体;它与大鼠室管膜细胞、视网膜Mueller细胞和软脑膜细胞的表面以及星形胶质细胞结合，但不与培养的神经元、少突胶质细胞、许旺细胞、小胶质细胞或各种非神经细胞结合。该单克隆抗体所确定的抗原是蛋白酶敏感的和大鼠特异性的，被命名为大鼠神经抗原-2（Ran-2）。分离的大鼠室管膜细胞和培养的大鼠Mueller细胞不表达其他神经细胞类型特异性标志物，例如，破伤风毒素受体、大鼠神经抗原-1（Ran-1）、半乳糖苷或胶质细胞酸性蛋白（GFAP）。这些细胞不表达IgG的细胞表面Fc受体、吞噬乳胶珠或产生可检测量的Thy-1或纤连蛋白糖蛋白。
Mice were immunized with enriched populations of cultured rat astrocytes; their spleen cells were fused with [mouse] NS-1 myeloma cells to generate antibody-secreting hybridomas. Two stable hybridoma clones were isolated which secrete monoclonal IgG2 antibodies that react with the surface of the great majority of rat astrocytes in culture. One of these antibodies was studied in indirect immunofluorescence assay; it binds to the surface of rat ependymal cells, retinal Mueller cells and leptomeningeal cells and to astrocytes, but not to cultured neurones, oligodendrocytes, Schwann cells, microglia or various non-neural cells. The antigen defined by this monoclonal antibody is protease-sensitive and rat-specific, it was designated rat neural antigen-2 (Ran-2). Isolated rat ependymal cells and cultured rat Mueller cells do not express other neural cell-type-specific markers, e.g., tetanus toxin receptors, rat neural antigen-1 (Ran-1), galactocerebroside or glial fibrillary acidic protein (GFAP). These cells do not express cell surface Fc receptors for IgG, phagocytose latex beads or make detectable amounts of the Thy-1 or fibronectin glycoproteins.