ROLE OF CHOLECYSTOKININ IN INDUCTION AND MAINTENANCE OF DIETARY PROTEIN-STIMULATED PANCREATIC GROWTH
ROLE OF CHOLECYSTOKININ IN INDUCTION AND MAINTENANCE OF DIETARY PROTEIN-STIMULATED PANCREATIC GROWTH
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DOI：
10.1152/ajpgi.1992.262.4.g740
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发表时间：
1992-04-01
影响因子：
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通讯作者：
MORISSET, J
中科院分区：
文献类型：
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作者：
GREEN, GM;JURKOWSKA, G;MORISSET, J
本文研究了胆囊收缩素（CCK）在高蛋白饮食刺激胰腺生长中的诱导和维持作用。将适应5%酪蛋白饮食的大鼠转换为70%酪蛋白饮食21天。CCK受体拮抗剂MK-329以2.5mg·kg-1·d-1 ip给药，从饲喂70%酪蛋白的第0天（第0天治疗）或第7天（中期治疗）开始，此后。另一组在饲喂70%酪蛋白7天后恢复至5%酪蛋白。饲喂70%酪蛋白显著刺激胰腺DNA、RNA、湿重、蛋白质含量和糜蛋白酶含量分别增加32%、87%、74%、216%和1,450%。中期MK-329给药比第0天给药更有效，完全逆转了胰腺重量和RNA含量的增加，部分逆转了蛋白质和胰凝乳蛋白酶含量的增加，对DNA含量无影响。恢复到5%酪蛋白迅速逆转胰腺参数的增加，DNA除外。结果表明，CCK是必不可少的诱导和维持膳食蛋白刺激的胰腺肥大。
The role of cholecystokinin (CCK) in induction and maintenance of pancreatic growth stimulated by a high-protein diet was investigated. Rats adapted to 5% casein diet were switched to 70% casein for 21 days. MK-329, a CCK receptor antagonist, was administered at 2.5 mg.kg-1.day-1ip, beginning on day zero (day zero treatment) or day 7 (midcourse treatment) of feeding 70% casein and thereafter. Another group was returned to 5% casein after 7 days of feeding 70% casein. Feeding 70% casein significantly stimulated increases of 32, 87, 74, 216, and 1,450% in pancreatic DNA, RNA, wet weight, protein content, and chymotrypsin content, respectively. Midcourse treatment with MK-329 was more effective than day zero treatment, and it completely reversed increases in pancreatic weight and RNA content, partially reversed increases in protein and chymotrypsin content, and had no effect on DNA content. Return to 5% casein rapidly reversed increases in pancreatic parameters, except for DNA. The results indicate that CCK is essential for induction and maintenance of dietary protein-stimulated pancreatic hypertrophy.