RECENT PROGRESS IN MULTIFOCAL MULTIPHOTON MICROSCOPY.
RECENT PROGRESS IN MULTIFOCAL MULTIPHOTON MICROSCOPY.
复制标题
多焦点多光子显微镜的最新进展。
DOI：
10.1142/s1793545812500186
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发表时间：
2012-07-01
影响因子：
2.5
通讯作者：
Gao BZ
中科院分区：
文献类型：
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作者：
Qu J;Liu L;Shao Y;Niu H;Gao BZ
多焦多光子显微镜（MMM）最近已成为生物医学中进行三维快速荧光成像的重要工具。MMM利用各种分束技术将近红外激光束分成多个小光束，在样品上产生多焦点阵列，进行并行多光子激发，然后用面阵探测器同时记录来自所有焦点的荧光信号，显著提高了多光子显微镜的成像速度，并允许高效率地使用激发光。在本文中，我们讨论了几个MMM设置使用不同的分束设备，包括Nipkow旋转盘，微透镜阵列，一组分束镜，或衍射光学元件（DOE）的功能。特别是，我们提出了我们最近的工作，使用空间光调制器（SLM）的MMM的发展。
Multifocal multiphoton microscopy (MMM) has recently become an important tool in biomedicine for performing three-dimensional fast fluorescence imaging. Using various beamsplitting techniques, MMM splits the near-infrared laser beam into multiple beamlets and produces a multifocal array on the sample for parallel multiphoton excitation and then records fluorescence signal from all foci simultaneously with an area array detector, which significantly improves the imaging speed of multiphoton microscopy and allows for high efficiency in use of the excitation light. In this paper, we discuss the features of several MMM setups using different beamsplitting devices, including a Nipkow spinning disk, a microlens array, a set of beamsplitting mirrors, or a diffractive optical element (DOE). In particular, we present our recent work on the development of an MMM using a spatial light modulator (SLM).