Effects of dissolved calcium and phosphorous on osteoblast responses.
Effects of dissolved calcium and phosphorous on osteoblast responses.
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DOI：
10.1563/0-742.1
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发表时间：
2005-01-01
期刊：
影响因子：
--
通讯作者：
Ong, J L
中科院分区：
文献类型：
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作者：
Ma, S;Yang, Y;Ong, J L
羟基磷灰石（HA）的溶解行为及其对初始细胞反应的影响具有重要的基础和临床意义。采用X射线衍射和傅里叶变换红外光谱对等离子喷涂HA涂层进行了表征。分别采用火焰原子吸收法和钼蓝络合比色法测定了等离子喷涂HA涂层3周内钙离子和无机磷离子的释放量。为了研究HA涂层溶解对成骨细胞反应的影响，将额外的Ca和Pi添加到细胞培养基中以模拟溶解浓度。人胚胎腭间充质细胞，成骨细胞前体细胞系，被用来评估生物反应，以提高钙和磷媒体超过2周。通过碱性磷酸酶特异性测定和1，25（OH）2维生素D3刺激的骨钙素产生来测量成骨细胞分化和矿化。涂层具有HA型结构。FTIR表明涂层上可能存在碳酸盐。溶出度研究表明Ca和Pi随时间持续增加。在细胞培养研究中，在细胞培养基中存在额外的Ca浓度时，成骨细胞分化增强。然而，在细胞培养基中的额外的Pi浓度被建议减慢成骨细胞分化和矿化。
The dissolution behavior of hydroxyapatite (HA) and its effect on the initial cellular response is of both fundamental and clinical importance. In this study, plasma-sprayed HA coatings were characterized by X-ray diffraction and Fourier transform infrared spectroscopy (FTIR). Calcium (Ca) and inorganic phosphorous (Pi) ions released from plasma-sprayed HA coatings within 3 weeks were measured by flame atomic absorption and colorimetrically molybdenum blue complex, respectively. To investigate the effect of dissolution of HA coatings on osteoblast response, additional Ca and Pi were added into the cell culture media to simulate the dissolution concentrations. Human embryonic palatal mesenchyme cells, an osteoblast precursor cell line, were used to evaluate the biological responses to enhanced Ca and Pi media over 2 weeks. Osteoblast differentiation and mineralization were measured by alkaline phosphatase-specific assay and 1,25 (OH)2 vitamin D3 stimulated osteocalcin production. The coatings exhibited an HA-type structure. FTIR indicated the possible presence of carbonates on the coatings. A dissolution study indicated a continual increase in Ca and Pi over time. In the cell culture study, enhanced osteoblast differentiation occurred in the presence of additional Ca concentration in the cell culture media. However, additional Pi concentration in the cell culture media was suggested to slow down osteoblast differentiation and mineralization.