Effects of a gerF (lgt) mutation on the germination of spores of Bacillus subtilis
Effects of a gerF (lgt) mutation on the germination of spores of Bacillus subtilis
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DOI：
10.1128/jb.186.10.2984-2991.2004
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发表时间：
2004-05-01
影响因子：
3.2
通讯作者：
Setlow, P
中科院分区：
文献类型：
--
作者：
Igarashi, T;Setlow, B;Setlow, P
识别触发枯草芽孢杆菌孢子萌发的个别营养素的膜结合受体的蛋白质之一含有用于将二酰基甘油添加到蛋白质的N末端附近的半胱氨酸残基的识别序列。B。枯草芽孢杆菌缺乏gerF（lgt）基因编码的前脂蛋白二酰基甘油转移酶表现出显着减缓发芽响应营养发芽如前所述，但gerF孢子与钙和吡啶二羧酸或与十二胺的混合物的发芽是正常的，因为是gerF孢子缺乏所有营养发芽受体的自发发芽。gerF突变对营养发芽的有害影响最高的GerA营养受体引发的发芽，并不那么（但仍然显着）GerB营养受体引发的发芽。然而，有一点，如果有的话，GerK营养受体介导的孢子萌发的影响。如从后者的结果预测的，用丙氨酸替换被认为添加到这些营养素受体的二酰基甘油的半胱氨酸残基对GerA受体功能有很大的影响，对GerB受体功能的影响较小，对GerK受体功能的影响很小（如果有的话）。
One of the proteins of the membrane-bound receptors that recognize individual nutrients that trigger germination of spores of Bacillus subtilis contains the recognition sequence for diacylglycerol addition to a cysteine residue near the protein's N terminus. B. subtilis spores lacking the gerF (lgt) gene that codes for prelipoprotein diacylglycerol transferase exhibited significantly slowed germination in response to nutrient germinants as found previously, but germination of gerF spores with a mixture of Ca2+ and dipicolinic acid or with dodecylamine was normal, as was the spontaneous germination of gerF spores lacking all nutrient germinant receptors. The deleterious effects of the gerF mutation on nutrient germination were highest on germination triggered by the GerA nutrient receptor and were less so (but still significant) on germination triggered by the GerB nutrient receptor. However, there was little, if any, effect on GerK nutrient receptor-mediated spore germination. As predicted from the latter results, replacement by alanine of the cysteine residue to which diacylglycerol is thought to be added to these nutrient receptors had a large effect on GerA receptor function, less effect on GerB receptor function, and little, if any, effect on GerK receptor function.