Morphology and phylogenies of two hypotrichous brackish-water ciliates from China, Neourostylopsis orientalis n. sp and Protogastrostyla sterkii (Wallengren, 1900) n. comb., with establishment of a new genus Neourostylopsis n. gen. (Protista, Ciliophora, Hypotrichia)
Morphology and phylogenies of two hypotrichous brackish-water ciliates from China, Neourostylopsis orientalis n. sp and Protogastrostyla sterkii (Wallengren, 1900) n. comb., with establishment of a new genus Neourostylopsis n. gen. (Protista, Ciliophora, Hypotrichia)
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DOI：
10.1099/ijs.0.049403-0
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发表时间：
2013-03-01
影响因子：
2.8
通讯作者：
Al-Rasheid, Khaled A. S.
中科院分区：
文献类型：
--
作者：
Chen, Xiangrui;Shao, Chen;Al-Rasheid, Khaled A. S.
本文研究了两种下毛纤毛虫 Neurostylopsis orientalis n. 的形态、脆弱性和小亚基 (SSU) rRNA 基因序列。 sp. 和 Protogastrostyla sterkii (Wallengren, 1900) n.梳子。 （基本名Gastrostyla sterkii），采自中国南方沿海水域。 Neurostylopsis orientalis n． sp。主要通过棕褐色皮质颗粒的排列、口膜、额叶和横蔓的数量以及中腹卷毛对的特征来诊断。 SSU rRNA基因系统发育强烈支持Neurostylopsis n.新属的建立。 gen.，其特征主要在于以下特征：额叶和横向纤毛明显分化，存在颊纤毛，两个额末纤毛，仅由中腹对组成且不超过细胞中点的中腹复合体，每侧多于一排边缘纤毛，源自每个亲本行内的单个原基，缺乏尾部纤毛。因此，需要两种新的组合：Neurostylopsis songi (Lei et al., 2005) n．梳子，和Neurostylopsis flavicana (Wang et al., 2011) n。梳子。此外，本研究还改进了后口病和无口口病的诊断。 Protogastrostyla sterkii (Wallengren, 1900) n.梳子。与同类Protogastrostyla pulchra的不同之处主要体现在体形、活体颊野与体长的比例以及分子数据上。基于目前的研究，我们得出结论，J.Gong等人收集的P.pulchra河口种群[Gong et al., J Eukaryot Microbiol (2007) 54, 468-478]是P. sterkii的一个种群。
This paper investigates the morphology, infraciliature and small-subunit (SSU) rRNA gene sequences of two hypotrichous ciliates, Neourostylopsis orientalis n. sp., and Protogastrostyla sterkii (Wallengren, 1900) n. comb. (basionym Gastrostyla sterkii), collected from coastal waters in southern China. Neourostylopsis orientalis n. sp. is diagnosed mainly by the arrangement of brownish cortical granules, the numbers of adoral membranelles and frontal and transverse cirri and the characteristics of its midventral cirral pairs. The SSU rRNA gene phylogeny strongly supports the establishment of the new genus Neourostylopsis n. gen., which is characterized mainly by the following features: frontal and transverse cirri clearly differentiated, buccal cirri present, two frontoterminal cirri, midventral complex composed of midventral pairs only and not exceeding the halfway point of the cell, more than one row of marginal cirri on each side which derive from individual anlagen within each parental row, caudal cirri lacking. Thus, two new combinations are required: Neourostylopsis songi (Lei et al., 2005) n. comb., and Neourostylopsis flavicana (Wang et al., 2011) n. comb. Additionally, improved diagnoses for both Metaurostylopsis and Apourostylopsis are supplied in this study. Protogastrostyla sterkii (Wallengren, 1900) n. comb. differs from the similar congener Protogastrostyla pulchra mainly in body shape, ratio of buccal field to body length in vivo and molecular data. Based on the present studies, we conclude that the estuarine population of P. pulchra collected by J. Gong and others [Gong et al., J Eukaryot Microbiol (2007) 54, 468-478] is a population of P. sterkii.