Long-Term Phosphorus Fertilization Impacts Soil Fungal and Bacterial Diversity but not AM Fungal Community in Alfalfa
Long-Term Phosphorus Fertilization Impacts Soil Fungal and Bacterial Diversity but not AM Fungal Community in Alfalfa
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DOI：
10.1007/s00248-009-9583-z
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发表时间：
2010-02-01
影响因子：
3.6
通讯作者：
St-Arnaud, M.
中科院分区：
文献类型：
--
作者：
Beauregard, M. S.;Hamel, C.;St-Arnaud, M.
土壤功能可能会受到影响土壤微生物群落的耕作措施的影响。在8年生苜蓿单作区，研究了不同施磷量（0、20和40 kg P2 O 5 ha（-1））对土壤微生物多样性的影响。采用变性梯度凝胶电泳和磷脂脂肪酸（PLFA）分析方法，研究了土壤细菌、真菌和丛枝菌根（AM）真菌的多样性。土壤肥力相关的参数（土壤磷酸盐通量，可溶性磷，水分，磷酸酶和脱氢酶测定，土壤有机质的轻组分的碳和氮含量）也进行了监测和相关的土壤微生物核糖型配置文件。土壤磷肥力的变化与施肥没有影响作物产量在8年，但在本研究的一年是与真菌和细菌群落的组成的变化，而不影响其丰富性，证明了检测到的核糖体的平均数量的影响的情况下。然而，差异施肥的历史所创造的土壤磷水平的变化并没有显着影响AM真菌核糖体组合，也没有AM真菌生物量与PLFA 16：1 Ω 5测量。脱氢酶测定结果表明，施肥增加了土壤磷通量和可溶性磷水平，但降低了土壤水分和土壤微生物活性。结果表明，土壤磷肥力管理可能会影响土壤微生物的土壤过程。季节变化也记录在微生物活性，土壤可溶性磷水平，以及在丰富的特定细菌和真菌PLFA土壤微生物生物量的指标。
Soil function may be affected by cropping practices impacting the soil microbial community. The effect of different phosphorus (P) fertilization rates (0, 20, or 40 kg P2O5 ha(-1)) on soil microbial diversity was studied in 8-year-old alfalfa monocultures. The hypothesis that P fertilization modifies soil microbial community was tested using denaturing gradient gel electrophoresis and phospholipids fatty acid (PLFA) profiling to describe soil bacteria, fungi, and arbuscular mycorrhizal (AM) fungi diversity. Soil parameters related to fertility (soil phosphate flux, soluble P, moisture, phosphatase and dehydrogenase assays, and carbon and nitrogen content of the light fraction of soil organic matter) were also monitored and related to soil microbial ribotype profiles. Change in soil P fertility with the application of fertilizer had no effect on crop yield in 8 years, but on the year of this study was associated with shifts in the composition of fungal and bacterial communities without affecting their richness, as evidenced by the absence of effect on the average number of ribotypes detected. However, variation in soil P level created by a history of differential fertilization did not significantly influence AM fungi ribotype assemblages nor AM fungi biomass measured with the PLFA 16:1 omega 5. Fertilization increased P flux and soil soluble P level but reduced soil moisture and soil microbial activity, as revealed by dehydrogenase assay. Results suggest that soil P fertility management could influence soil processes involving soil microorganisms. Seasonal variations were also recorded in microbial activity, soil soluble P level as well as in the abundance of specific bacterial and fungal PLFA indicators of soil microbial biomass.