Dielectric spectroscopy as a viable biosensing tool for cell and tissue characterization and analysis
Dielectric spectroscopy as a viable biosensing tool for cell and tissue characterization and analysis
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DOI：
10.1016/j.bios.2013.04.017
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发表时间：
2013-11-15
影响因子：
12.6
通讯作者：
Tabrizian, Maryam
中科院分区：
文献类型：
--
作者：
Heileman, Khalil;Daoud, Jamal;Tabrizian, Maryam
近年来，使用介电光谱学对细胞进行实时观察并提取有关其生理特性的丰富信息的方法得到了广泛的应用。这种受欢迎程度是由于介电光谱学的简单、易于使用、非侵入性和实时的性质。介电光谱学与微流控设备的轻松集成使这项技术进一步扩展到生物医学研究。介电频谱是通过向单元施加电信号来获得的，该电信号在一个频率范围内被扫描。本文综述了介电谱的不同解释方法以及阻抗谱在细胞观察中的应用进展。首先，讨论了获得细胞特定电学性质(细胞膜电容和细胞质电导率)的方法。这些电学性质是通过将介电谱拟合到不同的模型和方程来获得的。随后介绍了减小电双层影响的集成模型。然后讨论了阻抗平台，包括电子单元衬底阻抗传感(ECIS)。ECIS系统分为微电极阵列、交叉型电极和允许不同ECIS测量的电极。然后讨论了允许单电池和次单电池测量的平台。最后，阐述了阻抗谱在一系列细胞观察中的应用。这些应用包括观察细胞分化、有丝分裂、细胞周期和细胞毒性/细胞死亡。未来的应用，如药物筛选和在护理点应用，然后被涵盖。(C)Elsevier B.V.出版的2013年
The use of dielectric spectroscopy to carry out real time observations of cells and to extract a wealth of information about their physiological properties has expanded in recent years. This popularity is due to the simple, easy to use, non-invasive and real time nature of dielectric spectroscopy. The ease of integrating dielectric spectroscopy with microfluidic devices has allowed the technology to further expand into biomedical research. Dielectric spectra are obtained by applying an electrical signal to cells, which is swept over a frequency range. This review covers the different methods of interpreting dielectric spectra and progress made in applications of impedance spectroscopy for cell observations. First, methods of obtaining specific electrical properties of cells (cell membrane capacitance and cytoplasm conductivity) are discussed. These electrical properties are obtained by fitting the dielectric spectra to different models and equations. Integrating models to reduce the effects of the electrical double layer are subsequently covered. Impedance platforms are then discussed including electrical cell substrate impedance sensing (ECIS). Categories of ECIS systems are divided into microelectrode arrays, interdigitated electrodes and those that allow differential ECIS measurements. Platforms that allow single cell and sub-single cell measurements are then discussed. Finally, applications of impedance spectroscopy in a range of cell observations are elaborated. These applications include observing cell differentiation, mitosis and the cell cycle and cytotoxicity/cell death. Future applications such as drug screening and in point of care applications are then covered. (C) 2013 Published by Elsevier B.V.