Intracellular sodium and calcium in the postischemic myocardium.
Intracellular sodium and calcium in the postischemic myocardium.
复制标题
缺血后心肌细胞内的钠和钙。
DOI：
10.1016/s0003-4975(10)62818-4
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Feinberg,H
中科院分区：
文献类型：
--
作者：
Pridjian,AK;Levitsky,S;Krukenkamp,I;Silverman,NA;Feinberg,H
在30个离体灌注大鼠心脏暴露于从0到40分钟不等的常温缺血间隔，然后再灌注35分钟，细胞内钠和钙进行了测定。这些元素的积累与缺血后等容收缩功能的改变相关。分别在30、35和40分钟的缺血间隔后再灌注后，非缺血对照组的钙增加了207%、390%和681%。这些对应于30分钟后峰值发展压力下降23.8%，35分钟后下降68.6%，缺血40分钟后功能没有恢复。35分钟和40分钟的缺血分别使细胞内钠增加138%和170%。这些数据表明，存在与缺血持续时间和缺血后心脏功能损害程度相关的细胞内钠和钙的分级积累。因此，细胞内钠和钙的测量可以作为其他生化和功能因素的辅助手段，用于定量缺血后和心脏停搏后损伤，以开发新的心肌保护方案。
Intracellular sodium and calcium were measured in 30 isolated perfused rat hearts exposed to normothermic ischemic intervals varying from 0 to 40 minutes followed by 35 minutes of reperfusion. Accumulation of these elements was correlated with alterations in postischemic isovolumic contractile function. There were increases in calcium of 207%, 390%, and 681% of the nonischemic control following reperfusion after respective ischemic intervals of 30, 35, and 40 minutes. These corresponded to decrements in peak developed pressure of 23.8% after 30 minutes, 68.6% after 35 minutes, and no recovery of function after 40 minutes of ischemia. Thirty-five and 40 minutes of ischemia produced 138% and 170% increases in intracellular sodium, respectively. These data show that there is a graded accumulation of intracellular sodium and calcium that correlates with the duration of ischemia and the degree of functional impairment in the postischemic heart. Measurement of intracellular sodium and calcium may, therefore, serve as an adjunct to other biochemical and functional factors used to quantitate postischemic and postcardioplegic damage in the development of new myoprotective regimens.