FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells
FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells
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DOI：
10.1111/j.0022-2720.2004.01348.x
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发表时间：
2004-05-01
影响因子：
2
通讯作者：
Satiat-Jeunemaitre, B
中科院分区：
文献类型：
--
作者：
Bolte, S;Talbot, C;Satiat-Jeunemaitre, B
FM-染料被广泛用于研究活的真核细胞中的内吞作用、囊泡运输和细胞器组织。FM染料在植物细胞中的使用越来越多，引起了许多争论，关于它们是否适合作为内吞标记物，它们染色的细胞器以及它们通过囊泡运输网络遵循的精确途径。这篇文章的主要目的是批判性地评估这场辩论在植物细胞中的现状。为此，提供了关于FM染料的重要特性、最佳染料浓度、染料储存条件以及染色和成像方案的背景信息。特别强调的是放在使用FM-染料在双标记实验，以确定特定的细胞器。以这种方式，首次证明了用FM 4 -64染色高尔基体。
FM-dyes are widely used to study endocytosis, vesicle trafficking and organelle organization in living eukaryotic cells. The increasing use of FM-dyes in plant cells has provoked much debate with regard to their suitability as endocytosis markers, which organelles they stain and the precise pathways they follow through the vesicle trafficking network. A primary aim of this article is to assess critically the current status of this debate in plant cells. For this purpose, background information on the important characteristics of the FM-dyes, and of optimal dye concentrations, conditions of dye storage, and staining and imaging protocols, are provided. Particular emphasis is placed on using the FM-dyes in double labelling experiments to identity specific organelles. In this way, staining of the Golgi with FM4-64 has been demonstrated for the first time.