Enzymatic glucose sensors. Improved long-term performance in vitro and in vivo.
Enzymatic glucose sensors. Improved long-term performance in vitro and in vivo.
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DOI：
10.1097/00002480-199404000-00007
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发表时间：
1994-04-01
期刊：
影响因子：
--
通讯作者：
von Heimburg, D
中科院分区：
文献类型：
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作者：
Updike, S J;Shults, M C;von Heimburg, D
我们研究了酶电极葡萄糖传感器在体外和体内的长期性能。单一的商业生产的酶活性膜在体外14-36个月内仍然具有估计葡萄糖的功能。将这些膜皮下植入大鼠体内1年，经解释，仍具有体外测量葡萄糖的功能。具有这些膜的传感器加上具有较低葡萄糖渗透性的额外外膜允许在皮下组织的低氧张力下进行葡萄糖监测。这些传感器通过手术植入三只非糖尿病犬。将每个传感器植入物与无线电发射器耦合，以允许在这些清醒的未受限制的犬中进行连续长期葡萄糖监测。通过静脉内葡萄糖输注评价体内探头性能，并在临床实验室进行参考血糖测定。这些皮下植入的传感器跟踪血糖变化长达12周。三种传感器植入物的体内初始响应约为35秒（n = 8）。推注后传感器对葡萄糖的峰值响应范围为3至14分钟。在两只犬中证明了传感器灵敏度在+/- 15%范围内的稳定性超过1个月。传感器的寿命是有限的酶活性的损失，但最外层的聚氨酯膜的生物降解。研究结果表明，使用皮下植入的酶电极传感器长期连续监测血糖可能是可能的。
We studied the long-term in vitro and in vivo performance of enzyme electrode glucose sensors. Single commercially produced enzyme-active membranes remained functional for estimating glucose in vitro for 14-36 months. These membranes were implanted subcutaneously in rats for 1 year and, upon explanation, remained functional for measuring glucose in vitro. Sensors with these membranes plus an additional outer membrane with lower glucose permeability allowed glucose monitoring in the low oxygen tension of subcutaneous tissue. These sensors were surgically implanted in three nondiabetic dogs. Each sensor implant was coupled to a radio transmitter to allow continuous long-term glucose monitoring in these awake unrestrained dogs. In vivo sensor performance was evaluated by intravenous glucose infusion, with reference blood glucose determinations made in the clinical laboratory. These subcutaneously implanted sensors tracked changes in plasma glucose for up to 12 weeks. The in vivo initial response for three sensor implants was approximately 35 sec (n = 8). Sensor peak response to glucose after bolus infusion ranged from 3 to 14 min. Stability of sensor sensitivity within +/- 15% for more than 1 month was demonstrated in two of the dogs. Sensor lifetime was limited not by loss of enzyme activity, but by biodegradation of the outermost polyurethane membrane. The findings suggest that long-term continuous monitoring of blood glucose using a subcutaneously implanted enzyme electrode sensor may be possible.