Hotspot mutations in common oncogenes are infrequent in nasopharyngeal carcinoma.
Hotspot mutations in common oncogenes are infrequent in nasopharyngeal carcinoma.
复制标题
常见癌基因的热点突变在鼻咽癌中并不常见。
DOI：
10.3892/or.2014.3376
复制
发表时间：
2014-10
期刊：
影响因子：
4.2
通讯作者：
Ma Jun
中科院分区：
文献类型：
--
作者：
Jiang Ning;Liu Na;Yang Fan;Zhou Qiming;Cui Ruixue;Jiang Wei;He Qingmei;Li Wenfei;Guo Ying;Zeng Jing;Yun Jingping;Chen Xinchun;Zhou Boping;Sun Ying;Wang Huiyun;Chen Zhuo G;Ma Jun
癌基因突变有助于癌症的发生，可以为临床抗癌管理提供潜在的治疗靶点。然而，鼻咽癌（NPC）的癌基因突变模式尚未完全阐明。为了深入了解鼻咽癌的突变模式，研究人员使用高通量OncoCarta面板分析方法，在8种鼻咽癌细胞系和来自中国南方的160例鼻咽癌患者样本中确定了19种常见癌基因的238个热点突变。进一步进行统计分析，以确定癌基因突变与选定的临床病理特征之间的关系。总共，我们在17例（10.6%）鼻咽癌患者中发现了11种癌基因的24个突变。四名患者表现出至少一种致癌基因突变。我们还在7个NPC细胞系中发现了PIK3CA H1047R突变体。此外，癌基因突变与危险习惯（吸烟和饮酒）或除TNM分期外的其他临床特征均无相关性。KIT突变与较差的总生存率和无复发生存率相关。此外，KIT突变与年龄和N分期是鼻咽癌的独立预后因素。综上所述，本研究首次报道了鼻咽癌中多种癌基因的突变。我们发现热点癌基因突变在中国南方鼻咽癌患者中并不常见。由于缺乏热点突变，需要对鼻咽癌的基因突变进行全面的表征，以便在未来开发新的治疗靶点。
Oncogene mutations contribute to carcinogenesis and can provide potential therapeutic targets for clinical anticancer management. However, oncogene mutation patterns in nasopharyngeal carcinoma (NPC) have yet to be fully elucidated. To gain insight into mutation patterns in NPC, a high-throughput OncoCarta panel assay was used to determine 238 hotspot mutations across 19 common oncogenes in 8 NPC cell lines and 160 NPC patient samples from southern China. Statistical analyses were further conducted to identify associations between oncogene mutations and selected clinicopathological characteristics. In total, we identified 24 mutations across 11 oncogenes in 17 (10.6%) NPC patients. Four patients exhibited mutations in at least one oncogene. We also identified a PIK3CA H1047R mutant in 7 NPC cell lines. In addition, oncogene mutations showed no correlation with either risk habits (smoking and drinking) or other clinical characteristics except for TNM stage. KIT mutations were associated with poorer overall and relapse-free survival. Furthermore, KIT mutations together with age and N stage were independent prognostic factors in NPC. Taken together, the present study is the first report on mutations in multiple oncogenes in NPC. We found that hotspot oncogene mutations are infrequent in NPC patients from southern China. The lack of hotspot mutations requires a comprehensive characterization of gene mutations in NPC for developing new therapeutic targets in the future.
登录
查看更多内容
影响因子：
120.1
作者：
Daniel Jones
通讯作者：
Daniel Jones
影响因子：
--
作者：
Pei-yu Huang;M. Hong;Xing Zhang;H. Mai;D. Luo;Li Zhang
通讯作者：
Pei-yu Huang;M. Hong;Xing Zhang;H. Mai;D. Luo;Li Zhang
DOI：
10.1016/j.ypdi.2012.01.036
发表时间：
2012
期刊：
Yearbook of Pulmonary Disease
影响因子：
--
作者：
L. Tanoue
通讯作者：
L. Tanoue
影响因子：
11.5
作者：
Tie, Jeanne;Lipton, Lara;Sieber, Oliver M.
通讯作者：
Sieber, Oliver M.
影响因子：
45.3
作者：
Wee, J;Tan, EH;Machin, D
通讯作者：
Machin, D