VH‐gene expression in murine lipopolysaccharide blasts distributes over the nine known VH‐gene groups and may be random
VH‐gene expression in murine lipopolysaccharide blasts distributes over the nine known VH‐gene groups and may be random
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鼠脂多糖母细胞中的 VH 基因表达分布在九个已知的 VH 基因组中，并且可能是随机的
DOI：
10.1002/eji.1830151117
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发表时间：
1985
影响因子：
5.4
通讯作者：
K. Rajewsky
中科院分区：
文献类型：
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作者：
R. Dildrop;U. Krawinkel;E. Winter;K. Rajewsky
对脂多糖活化B细胞杂交瘤中VH基因的表达进行了分析。将分离的细胞质RNA与代表9个已知VH基因组的探针杂交或进行mRNA测序。在杂交瘤的收集中，所有9组的VH基因的表达频率在大多数情况下与群体的相对复杂性有相当好的相关性。在54份RNA样本中，有51份可以鉴定出VH基因转录本，并与已知的VH基因群之一相对应。因此，后者似乎本质上代表了小鼠的Vn基因簇。
VH‐gene expression in hybridomas derived from lipopolysaccharide‐activated B cells was analyzed. Isolated cytoplasmic RNA was hybridized to probes representing the 9 known VH‐gene groups or subjected to mRNA sequencing. In the collection of hybridomas VH genes of all 9 groups are expressed at frequencies which in most correlate reasonably well with the relative complexities of the groups. In 51 out of 54 RNA samples VH‐gene transcripts could be identified and corresponded to one of the known VH‐gene groups. It therefore appears that the latter essentially represent the Vn‐gene cluster of the mouse.
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
White,BA;Bancroft,FC
通讯作者：
Bancroft,FC