Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and dogs living in the same locality
Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and dogs living in the same locality
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DOI：
10.2307/3284315
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发表时间：
1997-02-01
影响因子：
1.3
通讯作者：
Thompson, RCA
中科院分区：
文献类型：
--
作者：
Hopkins, RM;Meloni, BP;Thompson, RCA
描述了一种基于聚合酶链反应的利用小亚基核糖体（SSU）RNA基因5'端附近的多态性区域对贾第鞭毛虫分离株进行基因分型的方法。使用直接从粪便中纯化的贾第虫包囊进行分析。分离物收集自生活在隔离的土著社区的人和狗，在那里Ginrrlin感染是高度流行的。这是第一次报告的遗传特征的贾第虫从狗和人类生活在同一地区。比较13个人和9个狗分离株的SSU-rRNA序列，发现4个不同的基因组。第1组和第2组包含所有人分离株。而第3组和第4组完全由从狗中回收的贾第虫样品组成。一个犬样品含有来自第2组和第3组的模板。这些结果表明，人与狗之间的贾第虫感染的人畜共患传播在这些社区并不频繁发生。与狗相关的SSU-rRNA序列未见报道，提示存在一个新的G.亚群9个体外培养分离株的序列分析支持了组间观察到的差异的遗传基础，这些分离株被置于通过酶电泳建立的相同遗传组中。
A polymerase chain reaction-based method for genotyping Giardia duodenalis isolates using a polymorphic region near the 5' end of the small subunit ribosomal (SSU) RNA gene is described. Analysis was performed using Giardia cysts purified directly from feces. isolates were collected from humans and dogs living in isolated Aboriginal communities where Ginrrlin infections are highly endemic. This is the first report of the genetic characterization of Giardia from dogs and humans living in the same locality. Comparison of the SSU-rRNA sequences from 13 human and 9 dog isolates revealed 4 different genetic groups. Groups 1 and 2 contained all of the human isolates. whereas groups 3 and 4 consisted entirely of Giardia samples recovered from dogs. One dog sample contained templates from both groups 2 and 3. These results suggest that zoonotic transmission of Giardia infections between humans and dogs does not occur frequently in these communities. The dog-associated SSU-rRNA sequences have not been reported before, suggesting a new G. duodenalis subgroup. A genetic basis for the differences observed between the groups was supported by sequence analysis of 9 in vitro cultured isolates that were placed into the same genetic groups established by enzyme electrophoresis.