Molecular marker-assisted backcrossing breeding: an example to transfer a thermostableβ-amylase gene from wild barley
Molecular marker-assisted backcrossing breeding: an example to transfer a thermostableβ-amylase gene from wild barley
复制标题
分子标记辅助回交育种：从野生大麦转移耐热β-淀粉酶基因的例子
DOI：
10.1007/s11032-018-0828-8
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发表时间：
2018
影响因子：
3.1
通讯作者：
Li CD
中科院分区：
文献类型：
--
作者：
Xu YH;Zhang XQ;Harasymow S;Westcott S;Zhang WY;Li CD
分子标记辅助回交（MABC）被广泛推荐用于将有利等位基因从供体转移到优良品种。问题仍然是，《人与生物圈公约》是否是开发有竞争力的商业品种的有效办法。在这里，我们说明了一个热稳定的β-淀粉酶等位基因Sd 3从野生大麦转移到商业大麦品种Gairkirk。选择优良品系用于遵循标准常规育种过程的区域作物品种试验。结果表明，Sd 3等位基因不仅增加酶的热稳定性，但显着提高糖化力，一个重要的麦芽品质性状。BC 1F 1个体对最终后代的综合农艺性状和品质性状有着根本性的影响，说明了在MABC回交早期进行筛选的重要性。BC_3F_3家系间存在足够的遗传变异，可用于其它麦芽品质和农艺性状的选择。10个具有改进的β-淀粉酶热稳定性的育种系也具有改进的产量和籽粒饱满度。选择具有改进的制麦质量和农艺性状的三个优良品系以提供亲本品系，从而掺入野生大麦等位基因用于育种商业品种。应该考虑一种新的策略，即利用标记辅助选择和回交从野生亲本中转移有利的等位基因。
Molecular marker-assisted backcrossing (MABC) is widely recommended for transferring favorable alleles from a donor to an elite variety. The question remains whether MABC is an effective approach to developing a competitive commercial variety. Here, we illustrate the transfer of a thermostable β-amylase allele Sd3 from wild barley into a commercial barley variety Gairdner. The elite lines were chosen for the Regional Crop Variety Test that followed a standard conventional breeding process. The results demonstrated that the Sd3 allele not only increased enzyme thermostability but dramatically enhanced diastatic power, an important malting quality trait. The BC1F1individuals had a fundamental impact on the comprehensive agronomic and quality traits of the final progenies, demonstrating the importance of screening at the early stage of backcrossing in MABC. There was sufficient genetic variation in the BC3F3families to select other malting quality and agronomic traits. Ten individual breeding lines with improved β-amylase thermostability also had improved yields and grain plumpness. Three elite lines with improved malting quality and agronomic traits were selected to provide a parental line to incorporate the wild barley allele for breeding a commercial variety. A new strategy should be considered that uses marker-assisted selection and backcrossing to transfer a favorable allele from a wild parent.