Sonogenetics is a non-invasive approach to activating neurons in Caenorhabditis elegans.
Sonogenetics is a non-invasive approach to activating neurons in Caenorhabditis elegans.
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DOI：
10.1038/ncomms9264
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发表时间：
2015-09-15
影响因子：
16.6
通讯作者：
Chalasani SH
中科院分区：
文献类型：
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作者：
Ibsen S;Tong A;Schutt C;Esener S;Chalasani SH
神经科学中的一个主要挑战是可靠地激活单个神经元，特别是那些位于大脑更深处的神经元。目前的光遗传方法需要侵入性的外科手术，将特定波长的光传递到靶细胞，以激活或沉默它们。在这里，我们演示了使用低压超声波作为非侵入性触发器来激活线虫线虫中特定的超声敏感型神经元。我们首先证明野生动物对低压超声波不敏感，需要充满气体的微泡来传导超声波。我们发现，神经元特异性的Trp-4的错误表达，机械转导通道的造孔亚单位，使神经元对超声波刺激敏感，导致行为输出。此外，我们使用这种方法来操纵感觉神经元和中间神经元的功能，并确定PVD感觉神经元在改变运动行为中的作用。我们认为，这种方法可以广泛应用于体内细胞功能的操纵。常见的光遗传方法需要外科手术将特定波长的光传递到靶细胞。在这里，作者演示了使用低压超声波作为非侵入性触发器来激活秀丽线虫的特定神经元，并发现机械转导通道Trp-4使细胞对超声波刺激敏感。
A major challenge in neuroscience is to reliably activate individual neurons, particularly those in deeper brain regions. Current optogenetic approaches require invasive surgical procedures to deliver light of specific wavelengths to target cells to activate or silence them. Here, we demonstrate the use of low-pressure ultrasound as a non-invasive trigger to activate specific ultrasonically sensitized neurons in the nematode, Caenorhabditis elegans. We first show that wild-type animals are insensitive to low-pressure ultrasound and require gas-filled microbubbles to transduce the ultrasound wave. We find that neuron-specific misexpression of TRP-4, the pore-forming subunit of a mechanotransduction channel, sensitizes neurons to ultrasound stimulus, resulting in behavioural outputs. Furthermore, we use this approach to manipulate the function of sensory neurons and interneurons and identify a role for PVD sensory neurons in modifying locomotory behaviours. We suggest that this method can be broadly applied to manipulate cellular functions in vivo. Common optogenetic approaches require surgical procedures to deliver light of specific wavelengths to the target cells. Here the authors demonstrate the use of low-pressure ultrasound as a non-invasive trigger to activate specific neurons in Caenorhabditis elegans and find that the mechanotransduction channel TRP-4 sensitizes cells to the ultrasound stimulus.