Graphene solution-gated field effect transistor DNA sensor fabricated by liquid exfoliation and double glutaraldehyde cross-linking
Graphene solution-gated field effect transistor DNA sensor fabricated by liquid exfoliation and double glutaraldehyde cross-linking
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DOI：
10.1016/j.carbon.2018.01.078
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发表时间：
2018-04
期刊：
影响因子：
10.9
通讯作者：
Zhongrong Wang;Yunfang Jia
中科院分区：
文献类型：
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作者：
Zhongrong Wang;Yunfang Jia
基于石墨烯的溶液门控场效应晶体管（G-SgFET）已经被广泛研究作为用于各种分析物的潜在生物传感器。与Hummers法、机械剥离法和外延法等制备石墨烯的方法相比，液态剥离石墨烯（LEG）是近年来发展起来的一种经济、高效的碳材料。然而，生物敏感性和基于LEG的SgFET（bioLEG-SgFET）尚未得到证实，因为LEG纳米片上的含氧基团缺乏，而LEG纳米片被认为能够接枝生物传感探针。在这里，我们描述了一种制造bioLEG-SgFET的方法，并首次展示了它们在单链DNA（ssDNA）检测中的生物应用。通过扫描电子显微镜、原子力显微镜、拉曼光谱和X射线光电子能谱对所制备的LEG上清液、沉积膜和功能化膜进行了全面的表征。此外，G-SgFET所具有的典型双极传输特性以及生物修饰G-SgFET所表现出的离子屏蔽效应也证明了该方法的可行性。BioLEG-SgFET作为传感器的性能通过检测DNA杂交来评估，作为概念验证，包括靶互补ssDNA（csDNA），错配和半匹配ssDNA。csDNA的检测限约为10 fM。因此，LEG将是G-SgFET中使用的传统石墨烯材料的有前途的替代品。
Graphene based solution-gated field effect transistors (G-SgFETs) have been extensively investigated as potential biosensors for various analytes. Liquid exfoliated graphene (LEG) is a recently developed economic and efficient carbon material, in comparison with graphene materials produced by other methods like Hummers, mechanical exfoliation and epitaxy. However, bio-sensitive and LEG based SgFETs (bioLEG-SgFETs) have not been demonstrated because of the deficiency in oxygen containing groups on LEG nano-sheets which are believed to be able to graft bio-sensing probes. Here, we describe a method for fabricating bioLEG-SgFETs and demonstrate their bio-application in single-strand DNA (ssDNA) detection, for the first time. The as-prepared LEG supernatant, deposited and functionalized films are thoroughly characterized by scanning electron microscopy, atom force microscopy, Raman and X-ray photoelectron spectroscopy. Furthermore, the feasibility of the proposed method is testified by the typical ambipolar transferring features possessed by G-SgFETs, as well as the ionic screening effect exhibited by the bio-modified G-SgFETs. BioLEG-SgFETs' performance acting as a sensor is evaluated by the detection of DNA hybridization, as proof-of-concept, which include target complementary ssDNA (csDNA), mismatched and half-matched ssDNA. The limit-of-detection for csDNA is about 10 fM. Accordingly, LEG would be a promising alternative for the traditional graphene materials used in G-SgFETs.