Cold adaptation overrides developmental regulation of sarcolipin expression in mice skeletal muscle: SOS for muscle-based thermogenesis?
Cold adaptation overrides developmental regulation of sarcolipin expression in mice skeletal muscle: SOS for muscle-based thermogenesis?
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DOI：
10.1242/jeb.119164
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发表时间：
2015-08-01
影响因子：
2.8
通讯作者：
Periasamy, Muthu
中科院分区：
文献类型：
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作者：
Pant, Meghna;Bal, Naresh C.;Periasamy, Muthu
新生小鼠比成年小鼠有更大的产热需求，可能需要额外的产热手段，而不是棕色脂肪组织（BAT）的既定机制。我们和其他人最近发现了一种新的骨骼肌为基础的产热介质称为肌磷脂（SLN），通过解偶联肌内质网Ca 2 +-ATP酶（SERCA）。此外，我们已经表明，SLN的表达下调，在新生儿发育的大鼠。本研究探讨了两个问题：（1）SLN在新生小鼠中的表达是否受到发育调控？以及（2）如果是这样，冷适应会超越这一点吗？我们的数据表明，SLN的表达是在新生儿早期阶段较高，并逐渐下调，在快速收缩骨骼肌。有趣的是，我们证明了新生小鼠的冷适应可以防止SLN表达下调。这一观察结果表明，SLN介导的产热作用可以在极端的生理需要期间在更大程度上招募，除了BAT。
Neonatal mice have a greater thermogenic need than adult mice and may require additional means of heat production, other than the established mechanism of brown adipose tissue (BAT). We and others recently discovered a novel mediator of skeletal muscle-based thermogenesis called sarcolipin (SLN) that acts by uncoupling sarcoendoplasmic reticulum Ca2+-ATPase (SERCA). In addition, we have shown that SLN expression is downregulated during neonatal development in rats. In this study we probed two questions: (1) is SLN expression developmentally regulated in neonatal mice?; and (2) if so, will cold adaptation override this? Our data show that SLN expression is higher during early neonatal stages and is gradually downregulated in fast twitch skeletal muscles. Interestingly, we demonstrate that cold acclimation of neonatal mice can prevent downregulation of SLN expression. This observation suggests that SLN-mediated thermogenesis can be recruited to a greater extent during extreme physiological need, in addition to BAT.