A mouse model of weight gain after nicotine withdrawal.
A mouse model of weight gain after nicotine withdrawal.
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DOI：
10.1016/j.bbrc.2021.12.064
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发表时间：
2021-12
影响因子：
3.1
通讯作者：
Katsushi Takeda;Daisuke Aotani;Yusuke Kuga;Tomoki Jinno;Tingting Guo;Kento Ogawa;Yuki Shimizu;Rei Hattori;Takashi Yagi;Hiroyuki Koyama;Shigenobu Matsumura;Hiromi Kataoka;Tomohiro Tanaka
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文献类型：
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作者：
Katsushi Takeda;Daisuke Aotani;Yusuke Kuga;Tomoki Jinno;Tingting Guo;Kento Ogawa;Yuki Shimizu;Rei Hattori;Takashi Yagi;Hiroyuki Koyama;Shigenobu Matsumura;Hiromi Kataoka;Tomohiro Tanaka
戒烟会增加体重。然而，其基本机制尚未完全了解。我们在这里报告建立一个小鼠模型，表现出增加的体重增加后，尼古丁戒断。高脂肪饮食喂养的小鼠用尼古丁输注两周，然后用渗透微型泵再用溶剂输注两周。体重在尼古丁停止后立即增加，并且显著高于继续使用尼古丁的小鼠。在戒烟的第一周内，转换为溶剂的小鼠比尼古丁继续给药的小鼠消耗更多的食物，而耗氧量相当。在下丘脑食欲中心观察到增食欲的刺豚鼠相关肽的表达升高。配对喂养实验表明，尼古丁戒断后体重增加加快是由于能量摄入增加。作为药理学干预功效的展示，给予毒蜥外泌肽-4，并在戒断尼古丁的小鼠中显示出对能量摄入和体重增加的有效抑制。我们目前的模型为研究戒烟后能量调节的变化提供了一个独特的平台。
Smoking cessation increases body weight. The underlying mechanisms, however, have not been fully understood. We here report an establishment of a mouse model that exhibits an augmented body weight gain after nicotine withdrawal. High fat diet-fed mice were infused with nicotine for two weeks, and then with vehicle for another two weeks using osmotic minipumps. Body weight increased immediately after nicotine cessation and was significantly higher than that of mice continued on nicotine. Mice switched to vehicle consumed more food than nicotine-continued mice during the first week of cessation, while oxygen consumption was comparable. Elevated expression of orexigenic agouti-related peptide was observed in the hypothalamic appetite center. Pair-feeding experiment revealed that the accelerated weight gain after nicotine withdrawal is explained by enhanced energy intake. As a showcase of an efficacy of pharmacologic intervention, exendin-4 was administered and showed a potent suppression of energy intake and weight gain in mice withdrawn from nicotine. Our current model provides a unique platform for the investigation of the changes of energy regulation after smoking cessation.