Biosynthesis and turnover of anandamide and other N-acylethanolamines in peritoneal macrophages
Biosynthesis and turnover of anandamide and other N-acylethanolamines in peritoneal macrophages
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DOI：
10.1016/s0014-5793(99)01226-0
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发表时间：
1999-10-01
期刊：
影响因子：
3.5
通讯作者：
Schmid, HHO
中科院分区：
文献类型：
--
作者：
Kuwae, T;Shiota, Y;Schmid, HHO
添加外源性多不饱和N-酰基乙醇胺（NAE），包括大麻素（20：4 n-6 NAE），通过大麻素受体引起各种生物学效应，而饱和和单不饱和NAE是无活性的。用Ca ~（2+）离子载体A23187处理完整小鼠腹腔巨噬细胞诱导花生四烯酸动员对NAE及其前体N-酰基磷脂酰乙醇胺的产生没有影响（N-酰基PE），乙醇胺通过其与内源性脂肪酸的N-酰化导致NAE合成增强，但该途径对花生四烯酸没有选择性，从含H-2 O-18的培养基中将O-18掺入NAE和N-酰基PE的酰胺羰基中，证明了两种脂质的快速组成性周转：（C）1999年欧洲生物化学学会联合会。
Addition of exogenous Polyunsaturated N-acylethanolamines (NAEs), including anandamide (20:4n-6 NAE), elicit a variety of biological effects through cannabinoid receptors, whereas saturated and monounsaturated NAEs are inactive. Arachidonic acid mobilization induced by treatment of intact mouse peritoneal macrophages with Ca2+ ionophore A23187 had no effect on the production of NAE or its precursor N-acylphosphatidylethanolamine (N-acyl PE), ethanolamine resulted in enhanced NAE synthesis by its N-acylation with endogenous fatty acids, but this pathway was not selective for arachidonic acid, Incorporation of O-18 from H-2 O-18-containing media into the amide carbonyls of both NAE and N-acyl PE demonstrated a rapid, constitutive turnover of both lipids: (C) 1999 Federation of European Biochemical Societies.