A 300 KILOBASE INTERVAL GENETIC-MAP OF RICE INCLUDING 883 EXPRESSED SEQUENCES
A 300 KILOBASE INTERVAL GENETIC-MAP OF RICE INCLUDING 883 EXPRESSED SEQUENCES
复制标题
DOI：
10.1038/ng1294-365
复制
发表时间：
1994-12-01
期刊：
影响因子：
30.8
通讯作者：
MINOBE, Y
中科院分区：
文献类型：
--
作者：
KURATA, N;NAGAMURA, Y;MINOBE, Y
我们构建了一个高分辨率的水稻遗传图谱，包含1,383个DNA标记，平均间隔为300个碱基（kb）。这些标记分布在沿着1，575 cM的12个连锁群上，包括883个cDNA、265个基因组DNA、147个随机扩增多态性DNA（RAPD）和88个其它DNA。cDNA来源于水稻根和愈伤组织，通过单次测序分析并搜索与已知蛋白质的相似性。近260个水稻新基因被鉴定和定位，基因组DNA和克隆的RAPD片段也被测序以产生STS。我们的图谱是植物中第一个有意义的基因表达图谱。它也是植物中可用的密集遗传图谱，并且是第一个由克隆序列数据全面支持的图谱。
We have constructed a high resolution rice genetic map containing 1,383 DNA markers at an average interval of 300 kilobases (kb). The markers, distributed along 1,575 cM on 12 linkage groups, comprise 883 cDNAs, 265 genomic DNAs, 147 randomly amplified polymorphic DNAs (RAPD) and 88 other DNAs. cDNAs were derived from rice root and callus, analysed by single-run sequencing and searched for similarities with known proteins. Nearly 260 rice genes are newly identified and mapped, and genomic DNA and cloned RAPD fragments were also sequenced to generate STSs. Our map is the first significant gene expression map in plants. It is also the densest genetic map available in plants and the first to be backed up comprehensively by clone sequence data.