Anoxybacillus pushchinensis gen. nov., sp nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus falvithermus comb. nov.
Anoxybacillus pushchinensis gen. nov., sp nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus falvithermus comb. nov.
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DOI：
10.1099/00207713-50-6-2109
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发表时间：
2000-11-01
影响因子：
2.8
通讯作者：
Laurinavichius, K
中科院分区：
文献类型：
--
作者：
Pikuta, E;Lysenko, A;Laurinavichius, K
一种新的严格厌氧，嗜碱。从粪肥样品（pH 6.8）中分离出中度嗜热、发酵、孢子形成细菌菌株K1（T）。细胞是革兰氏阳性的、直的、非运动的杆状物，其在37-66 ℃的温度（最佳在62 ℃）和8.0-10.5的pH范围（最佳在9.5-9.7）中生长。该菌以D-葡萄糖、蔗糖、D-果糖、D-海藻糖和淀粉为碳源和能源。它需要维生素，它的生长受到酵母提取物的刺激。主要代谢产物为H-2和乙酸。细胞过氧化氢酶阴性，可以将硝酸盐还原为亚硝酸盐。DNA的G+C含量为42.2mol%。根据表型特征和16 S rDNA测序及DNA-DNA杂交数据，菌株K1（T）（= DSM 12423（T）= ATCC 700785（T）= VKM B-2193（T））被指定为新属Anoxybacillus gen. nov.，作为新种的代表，普什中国厌氧芽孢杆菌新种“黄色热芽孢杆菌”菌株D.Y.，与K1 T株关系密切，命名为Anoxybacillusflavithermuscomb，nov.（模式株= d.y. (T)= DSM 2641（T））。
A new strictly anaerobic, alkaliphilic. moderately thermophilic, fermentative, spore-forming bacterium, strain K1(T), was isolated from manure samples (pH 6.8). Cells were Gram-positive, straight, non-motile rods that grew at temperatures of 37-66 degreesC (optimum at 62 degreesC) and in a pH range of 8.0-10.5 (optimum at 9.5-9.7). The bacterium fermented D-glucose, sucrose, D-fructose, D-trehalose and starch as carbon and energy sources. It required vitamins and its growth is stimulated by yeast extract. The major metabolic products were H-2 and acetate. Cells were catalase-negative and could reduce nitrate to nitrite. The G+C content of the DNA was 42.2 mol%. Based on the phenotypic properties and 16S rDNA sequencing and DNA-DNA hybridization data, strain K1(T) (= DSM 12423(T) = ATCC 700785(T) = VKM B-2193(T)) was assigned to the new genus Anoxybacillus gen. nov., as a representative of a new species, Anoxybacillus pushchinensis sp. nov. 'Bacillus flavothermus' strain d.y., which was found to be closely related to strain K1T, is described as Anoxybacillus flavithermus comb, nov. (type strain = d.y.(T) = DSM 2641(T)).