Oxidative Degradation of the Monolayer of 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphocholine (POPC) in Low-Level Ozone.
Oxidative Degradation of the Monolayer of 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphocholine (POPC) in Low-Level Ozone.
复制标题
低浓度臭氧中 1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱 (POPC) 单层的氧化降解。
DOI：
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发表时间：
2015
影响因子：
3.3
通讯作者：
S. Ye
中科院分区：
文献类型：
--
作者：
Lin Qiao;Aimin Ge;Yimin Liang;S. Ye
环境臭氧是大气中常见的污染物，具有极高的氧化能力，能急剧改变生物分子的结构和功能，对公众健康有害。然而，关于低浓度臭氧影响的知识在分子水平上仍然非常有限。在本研究中，系统地研究了广泛存在于许多生物系统中的1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱（POPC，16:0-18:1 PC）的单层及其与1,2-二棕榈酰-sn-甘油-3-磷脂酰胆碱（DPPC，16:0 PC）的二元混合单层。低水平臭氧环境 (20 ± 10 ppb)，通过 π-A 等温线、和频发生 (SFG) 振动光谱和原子力显微镜 (AFM) 进行分析。我们的结果表明，POPC 单层不稳定，油基链中的 C=C 部分会被低浓度臭氧选择性氧化。 POPC 中的氧化脂质最初保留下来，并将亲水部分重新定向到水表面，并逐渐溶解到水溶液中。使用不饱和脂质分子时应非常小心，以避免其在周围环境中可能被氧化。本研究扩展并加深了我们对分子水平上不饱和脂质氧化机制的认识。
Ambient ozone is a common pollutant in the atmosphere that has an extremely high oxidative ability, can dramatically change the structure and functionality of biomolecules, and is harmful to public health. However, the knowledge about the influence of low-level ozone is still very limited at a molecular level. In the present study, the monolayer of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC, 16:0-18:1 PC) as well as its binary mixed monolayer with 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC, 16:0 PC), which are widely found in many biological systems, have been systematically investigated in a low-level ozone environment (20 ± 10 ppb), by π-A isotherm, sum frequency generation (SFG) vibrational spectroscopy, and atomic force microscopy (AFM). Our results demonstrate that the POPC monolayer is unstable and the C═C moieties in the oleyl chain are selectively oxidized by the low-level ozone. The oxidized lipids from POPC initially remain and reorientate the hydrophilic portion to the water surface and gradually dissolve into the aqueous solution. One should take great caution when using unsaturated lipid molecules to avoid their possible oxidation in the ambient environment. The present study expands and deepens our insights into the oxidation mechanism of unsaturated lipids at a molecular level.
DOI：
10.1021/jp404087s
发表时间：
2013-08
期刊：
The journal of physical chemistry. A
影响因子：
--
作者：
Joscha Kleber;K. Lass;G. Friedrichs
通讯作者：
Joscha Kleber;K. Lass;G. Friedrichs
影响因子：
3.4
作者：
Runas KA;Malmstadt N
通讯作者：
Malmstadt N
影响因子：
4.1
作者：
Wynalda, Kelly M.;Murphy, Robert C.
通讯作者：
Murphy, Robert C.