NEOCORTICAL HISTOGENESIS IN NORMAL AND REELER MICE - A DEVELOPMENTAL-STUDY BASED UPON [H-3] THYMIDINE AUTORADIOGRAPHY
NEOCORTICAL HISTOGENESIS IN NORMAL AND REELER MICE - A DEVELOPMENTAL-STUDY BASED UPON [H-3] THYMIDINE AUTORADIOGRAPHY
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DOI：
10.1016/0165-3806(82)90141-9
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
CAVINESS, VS
中科院分区：
文献类型：
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作者：
CAVINESS, VS
在 reeler 突变小鼠中，新皮质主要神经元类别的相对位置是倒置的。在[胚胎日]E11和E17之间的新皮质细胞发生期间，每隔48小时形成的神经元被[3H]胸苷标记。通过放射自显影追踪标记细胞在迁移过程中和迁移后的位置。在正常动物和卷绕动物中，同时形成的群体同时到达新皮质。 E13之后，随着皮质板的出现，正常动物中的迁移细胞群上升到皮质板和边缘层的界面，在那里它们停留在狭窄的层流区中。相比之下，在卷取器中，迁移被阻止在皮层深处。迁移细胞似乎无法上升穿过先前群体占据的区域。迁移完成后，两种基因型的神经元都会固定在位置上，并且在未来皮质生长过程中其相对位置几乎不会发生变化。尽管卷轴中神经元的迁移和迁移后位置存在异常，但两种基因型中同时形成的细胞群产生了相同的神经元类别。
The relative positions of the principal neuronal classes of neocortex are inverted in the reeler mutant mouse. Neurons formed at 48-h intervals throughout the period of neocortical cytogenesis between [embryonic day] E11 and E17 are labeled by [3H]thymidine. The positions of the labeled cells during and subsequent to their migrations are traced by autoradiography. Simultaneously-formed cohorts reach the neocortex at the same time in normal and reeler animals. After E13, subsequent to the appearance of the cortical plate, cohorts of migrating cells in the normal animal ascend to the interface of the cortical plate and marginal layer where they come to rest in a narrow laminar zone. In reeler, by contrast, migration is arrested in the depths of the cortex. The migrating cell appears unable to ascent through the zone occupied by the preceding cohorts. At the completion of migration neurons of both genotypes become fixed in position and undergo little subsequent shift in their relative positions in the course of future cortical growth. Despite the anomaly of migrations and the post-migratory positions of neurons in reeler, cohorts of cells formed at the same time in the 2 genotypes give rise to the same neuronal classes.