Epidural perfusion cooling protection against protracted spinal cord ischemia in rabbits.
Epidural perfusion cooling protection against protracted spinal cord ischemia in rabbits.
复制标题
硬膜外灌注冷却对兔子长期脊髓缺血的保护作用。
作者：
I. Vanický;M. Marsala;J. Gálik;J. Mars̆ala
在兔脊髓缺血模型中评估改良的硬膜外冷却技术的保护作用。通过两个连通的椎管开口，用冷（5 ℃）等渗盐水持续灌注诱导缺血的腰椎节段周围的硬膜外腔。这个过程允许脊髓在缺血期间保持深度低温（中央灰质内< 15摄氏度）。实验分为常温缺血组（A组）和低温缺血组（B组）。每组包含三个亚组的动物，分别进行20、40或60分钟的主动脉结扎。他们的神经功能的结果进行了评估，长达48小时缺血后，组间差异进行了比较。缺血后2天，所有动物均通过经心灌注固定处死，并对其腰椎节段进行组织病理学检查。此外，在60分钟缺血的动物中，在手术干预期间和48小时后再次记录脊髓体感诱发电位。在常温动物中，检测到截瘫的高发生率：缺血20分钟后为40%，40分钟后为75%，60分钟后为100%。相比之下，所有的低温动物表现出完全的神经恢复，即使在60分钟的缺血。电生理学和组织学观察结果与神经学结果明显相关。结果表明，硬膜外灌注冷却产生的脊髓深部低温对兔脊髓持续缺血具有有效的保护作用。
The protective effect of a modified epidural cooling technique was assessed in a rabbit spinal cord ischemia model. The epidural space around the lumbar segments with induced ischemia was continually perfused with cold (5 degrees C) isotonic saline via two communicating spinal canal openings. This procedure allowed the spinal cord to be kept deeply hypothermic (< 15 degrees C within central gray matter) during the ischemic period. The animals were subjected to either normothermic ischemia (Group A) or hypothermic ischemia (Group B). Each group contained three subgroups of animals undergoing 20, 40, or 60 minutes of aortic ligation. Their neurological outcomes were evaluated up to 48 hours postischemia, and the intergroup differences were compared. Two days postischemia, all of the animals were sacrificed by transcardial perfusion-fixation and their lumbar segments were processed for histopathological examination. In addition, in animals with 60-minute ischemia, spinal somatosensory evoked potentials were recorded during surgical intervention and again after 48 hours. In the normothermic animals, a high incidence of paraplegia was detected: in 40% after 20 minutes of ischemia, in 75% after 40 minutes, and in 100% after 60 minutes. In contrast, all of the hypothermic animals exhibited full neurological recovery even after 60 minutes of ischemia. Both electrophysiological and histological observations clearly correlated with the neurological findings. The results suggest that deep spinal cord hypothermia produced by epidural perfusion cooling provides effective protection against protracted spinal cord ischemia in rabbits.
NMDA 拮抗剂可防止哺乳动物脊髓神经元的低温损伤和死亡。
影响因子：
4.2
作者：
Lucas,JH;Wang,GF;Gross,GW
通讯作者：
Gross,GW