Expression of neurotrophic factors and neuropeptide receptors by Langerhans cells and the Langerhans cell-like cell line XS52: further support for a functional relationship between Langerhans cells and epidermal nerves.
Expression of neurotrophic factors and neuropeptide receptors by Langerhans cells and the Langerhans cell-like cell line XS52: further support for a functional relationship between Langerhans cells and epidermal nerves.
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DOI：
10.1111/1523-1747.ep12337516
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发表时间：
1997-10
期刊：
影响因子：
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通讯作者：
H. Torii;Zengmin Yan;J. Hosoi;R. Granstein
中科院分区：
文献类型：
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作者：
H. Torii;Zengmin Yan;J. Hosoi;R. Granstein
表皮朗格汉斯细胞常与降钙素基因相关的多肽神经联系在一起。此外，在几种检测中，降钙素基因相关肽抑制朗格汉斯细胞的抗原提呈功能。为了进一步探索朗格汉斯细胞和神经具有功能关系的假设，进行了研究。为了研究郎格汉斯细胞是否可能产生影响神经细胞分化的因素，我们使用朗格汉斯细胞样细胞系XS52作为郎格汉斯细胞的替代品，并将其与浓缩到90%的朗格汉斯细胞进行比较。经脂多糖刺激的XS52细胞培养上清可诱导嗜铬细胞瘤细胞系PC12分化为交感神经元样细胞。脂多糖刺激的富集朗格汉斯细胞也是如此。用特异性中和抗血清对条件上清液进行预处理，结果表明，诱导分化的活性主要来源于白介素6，少量来源于神经生长因子和碱性成纤维细胞生长因子。逆转录聚合酶链式反应发现XS52细胞系XS52-4D、XS52-11D和XS52-8B三个克隆均表达IL-6mRNA，且内毒素可显著增强其表达。在XS52-4D和XS52-11D中检测到神经生长因子和碱性成纤维细胞生长因子的mRNA，而在XS52-8B中未检测到。这些神经营养因子在富集的朗格汉斯细胞中的表达与XS52-4D非常相似。为了检测郎格汉斯细胞是否可能表达神经源性多肽受体，采用逆转录聚合酶链式反应寻找垂体腺苷环化酶激活多肽的I型、II型和III型受体以及胃泌素释放肽受体。所有被检测的克隆以及丰富的朗格汉斯细胞都表达垂体腺苷环化酶激活多肽II型和III型，以及胃泌素释放肽受体。这些结果提示朗格汉斯细胞和神经之间存在双向信号传递；神经细胞可能通过分泌某些神经肽来调节朗格汉斯细胞的功能，而朗格汉斯细胞可能通过分泌白介素6或其他因素来促进神经的分化。
Epidermal Langerhans cells are frequently anatomically associated with calcitonin gene-related peptide-containing nerves. Furthermore, calcitonin gene-related peptide inhibits Langerhans cells antigen-presenting function in several assays. Studies were performed to further explore the hypothesis that Langerhans cells and nerves have a functional relationship. To examine whether Langerhans cells may produce factors that influence nerve cell differentiation, we utilized the Langerhans cell-like cell line XS52 as a surrogate for Langerhans cells and compared it with Langerhans cells enriched to 90%. Supernatants conditioned by lipopolysaccharide-stimulated XS52 cells were able to induce the differentiation of the pheochromocytoma line PC12 into sympathetic neuron-like cells. This was also the case with enriched Langerhans cells stimulated by lipopolysaccharide. Pretreatment of conditioned supernatants with specific neutralizing anti-sera indicated that most of the differentiation-inducing activity was due to interleukin-6 and a small amount was due to nerve growth factor and basic fibroblast growth factor. By reverse transcriptase polymerase chain reaction, three clones of the XS52 cell line, XS52-4D, XS52-11D, and XS52-8B, were found to express mRNA for interleukin-6 and expression was markedly augmented by lipopolysaccharide. mRNA for nerve growth factor and basic fibroblast growth factor was detected in XS52-4D and XS52-11D, but not in XS52-8B. The expression of these neurotrophic factors by enriched Langerhans cells was quite similar to that of XS52-4D. In order to examine whether Langerhans cells may express receptors for nerve-derived peptides, reverse transcriptase polymerase chain reaction was employed to look for pituitary adenylate cyclase activating polypeptide type I, type II, and type III, and gastrin-releasing peptide receptors. All clones examined, as well as enriched Langerhans cells, expressed pituitary adenylate cyclase activating polypeptide type II and type III, and gastrin-releasing peptide receptors. These results suggest bi-directional signalling between Langerhans cells and nerves; nerve cells may regulate Langerhans cell function by elaboration of certain neuropeptides whereas Langerhans cells may promote the differentation of nerves by elaboration of interleukin-6 and, possibly, other factors.