Temperature and pH Stimuli-Responsive System Delivers Location-Specific Antimicrobial Activity with Natural Products.
Temperature and pH Stimuli-Responsive System Delivers Location-Specific Antimicrobial Activity with Natural Products.
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DOI：
10.1021/acsabm.3c00588
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发表时间：
2024-01-15
影响因子：
4.7
通讯作者：
Raval, Rasmita
中科院分区：
文献类型：
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作者：
Morris, Gareth;Goodman, Sean;Sorzabal Bellido, Ioritz;Milanese, Chiara;Girella, Alessandro;Pallavicini, Piersandro;Taglietti, Angelo;Gaboardi, Mattia;Jackel, Frank;Diaz Fernandez, Yuri A;Raval, Rasmita
具有可控刺激响应功能的智能材料处于技术发展的前沿。在这项工作中，我们提出了一种通用策略，该策略结合了简单的组件，物理化学反应和简单的制造方法，以实现能够特定位置的抗菌货物递送的双重刺激响应系统。该封装系统是通过结合聚二甲基硅氧烷（PDMS）的生物相容性惰性聚合物基质和饱和脂肪酸的生物活性货物来制造的。我们证明了我们的方法提供抗微生物活性的模式细菌大肠杆菌的有效性。该系统响应两个控制变量，温度和pH值，在不同的刺激组合下提供两种水平的抗菌反应：一种是对浮游介质的反应，另一种是直接在表面对固定细菌的反应。空间分辨拉曼光谱以及热分析和结构材料分析表明，该系统不仅表现出开/关状态，而且还可以控制活性物质向表面或液体介质的重新定位和靶向，从而导致浮游细菌和固定化细菌的不同开/关状态。本文提出的方法在技术上简单且可扩展，通过使用经批准的组件和依赖基本化学过程，在食品和医疗保健部门面临较低的监管障碍。我们的研究结果还提供了一个概念验证平台，用于设计和简单制造能够作为布尔逻辑门运行的输送系统，在不同的环境条件下提供不同的响应。
Smart materials with controlled stimuli-responsive functions are at the forefront of technological development. In this work, we present a generic strategy that combines simple components, physicochemical responses, and easy fabrication methods to achieve a dual stimuli-responsive system capable of location-specific antimicrobial cargo delivery. The encapsulated system is fabricated by combining a biocompatible inert polymeric matrix of poly(dimethylsiloxane) (PDMS) and a bioactive cargo of saturated fatty acids. We demonstrate the effectiveness of our approach to deliver antimicrobial activity for the model bacteria Escherichia coli. The system responds to two control variables, temperature and pH, delivering two levels of antimicrobial response under distinct combinations of stimuli: one response toward the planktonic media and another response directly at the surface for sessile bacteria. Spatially resolved Raman spectroscopy alongside thermal and structural material analysis reveals that the system not only exhibits ON/OFF states but can also control relocation and targeting of the active cargo toward either the surface or the liquid media, leading to different ON/OFF states for the planktonic and sessile bacteria. The approach proposed herein is technologically simple and scalable, facing low regulatory barriers within the food and healthcare sectors by using approved components and relying on fundamental chemical processes. Our results also provide a proof-of-concept platform for the design and easy fabrication of delivery systems capable of operating as Boolean logic gates, delivering different responses under different environmental conditions.