The intraepidermal sweat-duct unit is derived from cells of the dermal sweat-duct: direct evidence obtained from epithelial allografts
The intraepidermal sweat-duct unit is derived from cells of the dermal sweat-duct: direct evidence obtained from epithelial allografts
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表皮内汗管单位源自真皮汗管细胞：从上皮同种异体移植物获得的直接证据
DOI：
10.1007/bf00412632
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发表时间：
2004
影响因子：
3
通讯作者：
J. Thivolet
中科院分区：
文献类型：
--
作者：
Jean Kanitakis;G. Mauduit;M. Faure;J. Thivolet
表皮内汗腺导管单位（或称顶鞘）是一种独特的表皮结构，具有独特的形态和功能特性，使其与邻近的表皮区分开来。最近，已经表明AS也具有显著的抗原特性。例如，AS细胞是表皮中唯一在正常条件下表达HLA-DR [5，8]和癌胚抗原（CEA）[9]的上皮细胞。Christophers和Plewig [1]对人体AS的形成进行了详细研究，他们发现AS来源于真皮汗管（SD）远端部分的细胞。在这项工作中，我们观察了通过表皮细胞培养获得的人上皮同种异体移植物（EAG）中AS的重建。免疫组化数据清楚地表明，新形成的AS开发的表皮细胞的EAG，但从真皮SD细胞。根据绿色[3]的方法制备培养的上皮片（CES）。这些用于覆盖非免疫抑制成人患者的断层移植区域，这些患者的腿部溃疡采用传统的网状自体移植物进行治疗。尽管HLA和ABO血型抗原完全不匹配，但在随访期间（12个月），EAG未发生排斥反应[10，11]。CES和活检标本的EAG，采取在不同的时间间隔移植后，进行了研究，通过光镜和电镜和免疫组化技术。后者包括CEA、HLA-DR和ABO血型抗原的免疫标记。
The intraepiderma [sweat-duct unit (or acrosyringlum)(AS) represents a unique epithelial cutaneous structure with distinct morphological and functional properties that differentiate it from the neighboring epidermis. More recently, it has been shown that the AS also has remarkable antigenic properties. AS cells, for instance, are the only epithelial cells in the epidermis that, under normal conditions, express HLA-DR [5, 8] and carcinoembryonic antigen (CEA)[9]. Formation of the AS in man has been studied elaborately by Christophers and Plewig [1], who showed the AS to derive from cells of the distal portion of the dermal sweat duct (SD). In this work, we observed the reconstruction of AS in human epithelial allografts (EAG) obtained through cultures of epidermal cells. Immunohistochemical data clearly showed that the newly formed AS developed not from epidermal cells of the EAG but from dermal SD cells. Cultured epithelial sheets (CES) were produced according to the method of Green [3]. These were used to cover the split-thickness graft-taking area in nonimmunosuppressed adult patients whose leg ulcers were treated with conventional mesh autografts. Despite a complete mismatch for HLA and ABO bloodgroup antigens, the EAG were not rejected during the follow-up period (12 months)[10, 11]. The CES and biopsy specimens of the EAG, taken at various time intervals after grafting, were studied by light and electron microscopy and by immunohistochemical techniques. The latter included immunotabelling for CEA, HLA-DR and ABO blood-group antigens.