Fibroblast heterogeneity in the periodontium and other tissues
Fibroblast heterogeneity in the periodontium and other tissues
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DOI：
10.1111/j.1600-0765.1997.tb01398.x
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发表时间：
1997-01-01
影响因子：
3.5
通讯作者：
Blieden, TM
中科院分区：
文献类型：
--
作者：
Phipps, RP;Borrello, MA;Blieden, TM
成纤维细胞是居住在牙周组织中的主要细胞。因此，它们对维持支撑和固定牙齿的结缔组织至关重要。对于它们的起源、健康和疾病中调节细胞因子和生长因子的合成以及在软组织再生中的重要性，人们知之甚少。一个新兴的概念是，成纤维细胞不是同质的，而是由可以调节骨髓来源细胞（如T淋巴细胞）的细胞亚群组成的。根据成纤维细胞的形态、大小、中间纤维的表达以及胶原的亚型，可以将成纤维细胞分为不同的亚群。不同的表面标记表达也是区分成纤维细胞亚群与许多组织的关键特征。诸如Thy-1、II类MHC和Clq等表面蛋白已被成功地用于分离成纤维细胞。重要的是，这些成纤维细胞亚群不仅抗原性多样，而且具有不同的功能。Thy 1（-）肺成纤维细胞可以显示II类MHC抗原，合成IL-1，并能激活T淋巴细胞，而Thy 1（+）亚群缺乏这些功能。最近，来自人类眼眶的成纤维细胞也被证明是可分离的，基于the1表面标记的表达。来源于人类牙龈和牙周韧带的成纤维细胞似乎也由具有可遗传的表面标记模式的亚群组成，这将允许它们分离成功能亚群。本文将对牙周组织和其他组织中成纤维细胞异质性的研究结果进行综述。证据将提出使用表面标记来描绘功能子集。区分成纤维细胞亚群的能力将有助于牙周病发病机制和伤口愈合的研究。
Fibroblasts are the major resident cells which inhabit the periodontal tissues. As such, they are crucial for maintaining the connective tissues which support and anchor the tooth. Little is known of their origins, synthesis of regulatory cytokines and growth factors in health and disease, and importance in soft tissue regeneration. An emerging concept is that fibroblasts are not homogeneous, but instead consist of subsets of cells which can regulate bone marrow-derived cells such as T lymphocytes. Fibroblasts can be separated into subsets on the basis of morphology, size and expression of intermediate filaments as well as collagen subtypes. Differential surface marker expression has also been a key feature to distinguish fibroblast subsets from many tissues. Antigens such as Thy-1, class II MHC, and Clq are among those surface proteins which have been employed successfully to separate fibroblasts. Importantly, these fibroblast subsets are not only antigenically diverse, but also possess distinct functions. Thy 1(-) pulmonary fibroblasts can display class II MHC antigens, synthesize IL-1 and can activate T lymphocytes, whereas the Thy 1(+) subset is devoid of these functions. Recently, fibroblasts from the human orbit have also been shown to be separable on the basis of Thy 1 surface marker expression. Fibroblasts derived from human gingiva and periodontal ligament also appear to be composed of subsets with a heritable pattern of surface markers which will permit their separation into functional subpopulations. This paper will review findings of fibroblast heterogeneity in periodontal and other tissues. Evidence will be presented for the use of surface markers to delineate functional subsets. The ability to discriminate subsets of fibroblasts will aid in studies of periodontal disease pathogenesis and wound healing.