Aberrant expression of Eag1 potassium channels in gastric cancer patients and cell lines
Aberrant expression of Eag1 potassium channels in gastric cancer patients and cell lines
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DOI：
10.1007/s12032-007-0015-y
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发表时间：
2007-01-01
期刊：
影响因子：
3.4
通讯作者：
Ai, Yao-Wei
中科院分区：
文献类型：
--
作者：
Ding, Xiang-wu;Luo, He-Sheng;Ai, Yao-Wei
最近，钾离子通道和癌症之间形成了一种有趣的关系。本研究旨在探讨Eag1钾通道在胃癌组织中的表达及其在癌细胞生长中的作用。采用免疫组化和逆转录聚合酶链反应研究Eag1在胃癌患者、胃癌细胞系及胃腺瘤组织中的表达情况。此外，使用丙咪嗪鉴定eagle参与SGC-7901和BGC-823细胞的生长。胃癌原发组织中Eag1蛋白阳性表达频率为70.5% (67/95)，Eagl mRNA阳性表达频率为68.2%（15/22）。Eagl mRNA在两种胃细胞系中均呈阳性表达。在配对的非癌配对组织和5例腺瘤组织中，Eagl蛋白和mRNA均呈负表达。Eag1蛋白表达水平与淋巴结转移（P = 0.049）和分期（P = 0.039）相关，与性别、年龄、分化程度及其他器官转移无关。细胞计数和MTT法检测丙咪嗪对SGC-7901和BGC-823细胞在12 h和24 h的增殖有显著抑制作用（P < 0.01）。本研究表明，Eag1在胃癌组织细胞系中异常表达，与胃癌淋巴结转移和分期有关，在胃癌细胞的增殖中起重要作用。
Recently, an interesting relationship between potassium channels and cancer has evolved. The aim of this study is to investigate expression of Eag1 potassium channel in gastric cancer and its role in cancer cells growth. The expression of Eag1 for gasric cancer patients and cell lines as well as gastric adenoma was investigated by immunohistochemistry and reverse transcription polymerase chain reaction. In addition, imipramine was used to identify the involvement of Eagl in the growth of SGC-7901 and BGC-823 cells. Frequency of positive expression of Eag1 protein was 70.5% (67/95) and Eagl mRNA was 68.2% (15/22) in gastric cancer primary tissues. Eagl mRNA was positively expressed in two gastric cell lines. Eagl protein and mRNA were negatively expressed in paired non-cancerous matched tissues and 5 cases of adenoma tissues. The expression level of Eag1 protein was associated with lymph node metastasis (P = 0.049) and stage (P = 0.039), but had no correlation with sex, age, differentiation grades, and other organs metastases. Imipramine significantly inhibited the proliferation of SGC-7901 and BGC-823 cells at 12 h and 24 h detected by cells number counting and MTT assay (P < 0.01). The study indicates Eag1 is aberrantly expressed in gastric cancer tissues and cell lines and associated with cancer lymph node metastasis and stage and play an important role in the proliferation of gastric cancer cells.