Differences in mitochondrial genome organization of Cryptococcus neoformans strains
Differences in mitochondrial genome organization of Cryptococcus neoformans strains
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DOI：
10.1007/s10482-005-8544-x
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发表时间：
2005-10-01
影响因子：
2.6
通讯作者：
Kucsera, J
中科院分区：
文献类型：
--
作者：
Litter, J;Keszthelyi, A;Kucsera, J
组织的线粒体基因组在两个菌株属于不同品种的新生隐球菌进行了分析。IFM5844（var. neoformans）和IFO 410（var. grubii）菌株，通过杂交、克隆和测序构建功能图谱。在线粒体功能（ND 1、ND 2、ND 3、ND 4、ND 4L、ND 5、ND 6、ATP 6、ATP 9、COX 1、COX 2和COB）和蛋白质合成（SsrRNA和LsrRNA）中重要的大多数基因被定位。我们在这些基因的顺序上没有发现菌株之间的任何差异。然而，它们的mtDNA的大小有显著差异：IFM 5844为32.6 kb，IFO 410为24.1 kb。这可以归因于mtDNA的两个大区域。在这些区域中，发现COX 1的内含子数量存在差异（var. grubii，var. neoformans）、COB（var. Grubii，var. neoformans）、LsrRNA（var. Grubii，var. neoformans）和ND 5（var. Grubii，var. neoformans）基因。在COB和COX 1基因的多个内含子中发现了LAGLIDADG基序，部分基因的核苷酸序列以及基因间区的大小和序列也存在差异。将IFM和IFO株的基因核苷酸序列与来自数据库的H-99和JEC 21株的基因核苷酸序列进行比较。令人惊讶的高相似性被发现之间的菌株属于变种。grubii（IFO 410和H-99）和var.新生儿（IFM 5844和JEC 21）。
The organization of the mitochondrial genomes in two strains belonging in different varieties of Cryptococcus neoformans was analysed. Physical maps of the mtDNA of the IFM5844 (var. neoformans) and IFO410 (var. grubii) strains were constructed by using EcoRI and EcoRV restriction enzymes; functional maps were constructed by hybridization, cloning and sequencing. Most of the genes important in the mitochondrial function (ND1, ND2, ND3, ND4, ND4L, ND5, ND6, ATP6, ATP9, COX1, COX2 and COB) and protein synthesis (SsrRNA and LsrRNA) were localized. We did not find any differences between the strains in the order of these genes. However, they differed significantly in the sizes of the mtDNAs: 32.6 kb for IFM5844, and 24.1 kb for IFO410. This can be attributed to two large regions of the mtDNA. In these regions, differences were found in the numbers of introns in COX1 (no intron in var. grubii, 5 introns in var. neoformans), COB (1 intron in var. grubii, 2 introns in var. neoformans), LsrRNA (no intron in var. grubii, 2 introns in var. neoformans), and ND5 (no intron in var. grubii, 1 intron in var. neoformans) genes. In several introns of the COB and COX1 genes LAGLIDADG motifs were found. Differences were also observed in the nucleotide sequences of some genes and in the sizes and sequences of intergenic regions. The nucleotide sequences of the genes of the IFM and IFO strains were compared with those of the H-99 and JEC 21 strains from the database. Surprisingly high similarities were found between the strains belonging in var. grubii (IFO 410 and H-99) and var. neoformans (IFM 5844 and JEC 21).