Evaluation of the accuracy of hTERT gene aberrant methylation using electrochemical hybridization assay and liquid-based cytology in screening for oral squamous cell carcinoma
Evaluation of the accuracy of hTERT gene aberrant methylation using electrochemical hybridization assay and liquid-based cytology in screening for oral squamous cell carcinoma
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DOI：
10.1002/elan.202200564
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发表时间：
2023-04-07
期刊：
影响因子：
3
通讯作者：
Takenaka，Shigeori
中科院分区：
文献类型：
--
作者：
Haraguchi，Kazuya;Sato，Shinobu;Takenaka，Shigeori
为了提高口腔鳞状细胞癌（oral squamous cell carcinoma， OSCC）患者的生存率，需要一种可靠的、微创的、减轻患者负担的、具有高灵敏度和特异性的早期OSCC诊断方法。因此，我们使用三种类型的DNA探针固定化电极，采用基于二茂铁-二亚胺（FND）的电化学杂交试验（FND - EHA），在口腔脱落细胞的hTERT启动子区域的三个位置检测异常甲基化。我们还使用口腔脱落细胞进行了液体细胞学检查，并比较了这些获得的数据，以评估FND‐EHA是否可以用作OSCC筛查系统。结果表明，该方法与常规OSCC筛查和细胞学检查具有良好的相关性。此外，FND‐EHA还能够检测液体细胞学检测结果不明确的样品。这表明FND‐EHA可能是一种有用的OSCC筛选系统。
In order to improve the survival rate of oral squamous cell carcinoma (OSCC) patients, a reliable diagnostic method for early OSCC detection is required that is minimally invasive, less burdensome to the patient, and has high sensitivity and specificity. Therefore, we performed the detection of abnormal methylation at three locations in the hTERT promoter region of oral exfoliated cells by employing the ferrocenylnaphthalene diimide (FND)‐based electrochemical hybridization assay (FND‐EHA) using three types of DNA probe‐immobilized electrodes. We also performed liquid cytology using oral exfoliated cells and compared these obtained data to evaluate whether FND‐ EHA can be used as an OSCC screening system. The results showed a good correlation between this method and conventional OSCC screening, and cytology. In addition, FND‐EHA was also able to determine samples that had been ambiguously determined by liquid cytology. This indicates that FND‐EHA may be useful as an OSCC screening system.