Deletion of rpoB reveals a second distinct transcription system in plastids of higher plants
Deletion of rpoB reveals a second distinct transcription system in plastids of higher plants
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DOI：
10.1002/j.1460-2075.1996.tb00640.x
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发表时间：
1996-06-03
期刊：
影响因子：
11.4
通讯作者：
Maliga, P
中科院分区：
文献类型：
--
作者：
Allison, LA;Simon, LD;Maliga, P
高等植物中的质体基因组编码大肠杆菌样RNA聚合酶的亚基，该聚合酶从类似于大肠杆菌sigma（70）型启动子的序列启动质体基因的转录。通过删除烟草大肠杆菌样RNA聚合酶的必需β亚基的基因，我们已经建立了不利用大肠杆菌样启动子的第二个质体转录系统的存在。与大肠杆菌样RNA聚合酶相反，新的转录机制优先转录遗传系统基因，而不是光合基因。虽然突变体植物是光合缺陷，转录由这种聚合酶是足够的质体维护和植物发育。
The plastid genome in higher plants encodes subunits of an Escherichia coli-like RNA polymerase which initiates transcription of plastid genes from sequences resembling E.coli sigma(70)-type promoters. By deleting the gene for the essential beta subunit of the tobacco E.coli-like RNA polymerase, we have established the existence of a second plastid transcription system which does not utilize E.coli-like promoters. In contrast to the E.coli-like RNA polymerase, the novel transcription machinery preferentially transcribes genetic system genes rather than photosynthetic genes. Although the mutant plants are photosynthetically defective, transcription by this polymerase is sufficient for plastid maintenance and plant development.