Maternal docosahexaenoic acid‐enriched diet prevents neonatal brain injury
Maternal docosahexaenoic acid‐enriched diet prevents neonatal brain injury
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母亲富含二十二碳六烯酸的饮食可预防新生儿脑损伤
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Toshiaki Shimizu
中科院分区：
文献类型：
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作者：
H. Suganuma;Yasuhiro Arai;Y. Kitamura;M. Hayashi;A. Okumura;Toshiaki Shimizu
新生儿窒息引起的缺血性脑病是神经发育延迟的最重要原因之一。神经元凋亡延长在神经元变性过程中起重要作用。二十二碳六烯酸（DHA）是脑膜磷脂的主要成分，可防止神经元细胞凋亡，并作为抗氧化剂发挥重要作用。我们在新生儿缺氧缺血性脑病模型中研究了妊娠期间母亲补充DHA的神经保护和抗氧化作用。怀孕大鼠被随机分配到两个实验组：对照组或富含DHA的饮食组。通过左颈总动脉闭塞并暴露于8%氧气1.5小时产生缺血性脑病。 在出生后第8、10和14天进行TUNEL测定、caspase-3和8-羟基脱氧鸟苷（8-OHdG）的免疫组织化学和caspase-3的Western印迹。在出生后第7天估计脑的脂肪酸组成。母亲的饮食明显影响脑脂肪酸组成的幼崽。在富含DHA的组中，凋亡神经元细胞和8-OHdG免疫反应性显著减少。我们的研究结果表明，母亲在怀孕期间富含DHA的饮食通过抑制氧化应激和凋亡性神经元死亡提供神经保护。因此，怀孕期间膳食补充DHA可能有助于预防新生儿脑损伤。
Hypoxic‐ischemic encephalopathy due to neonatal asphyxia is one of the most important causes of delayed neurological development. Prolonged neuronal apoptosis plays an important role in the processes contributing to neuronal degeneration. Docosahexaenoic acid (DHA), a major component of brain membrane phospholipids, prevents neuronal cell apoptosis and plays an important role as an anti‐oxidant agent. We investigated the neuroprotective and anti‐oxidant effects of maternal DHA supplementation during pregnancy in a model of neonatal hypoxic‐ischemic encephalopathy. Pregnant rats were randomly assigned to two experimental groups: a control group or a DHA‐enriched diet group. Hypoxic‐ischemic encephalopathy was produced by left common carotid artery occlusion and exposure to 8% oxygen for 1.5 h. TUNEL assay, immunohistochemistry for caspase‐3 and 8‐hydroxy‐deoxyguanosine (8‐OHdG), and Western blot for caspase‐3 were performed at postnatal days 8, 10 and 14. Fatty acid composition of brain was estimated on postnatal day 7. Maternal diet clearly influenced brain fatty acid composition in pups. Numbers of apoptotic neuronal cells and 8‐OHdG immunoreactivity were significantly decreased in the DHA‐enriched group. Our findings indicate that maternal DHA‐enriched diet during pregnancy provides neuroprotection by inhibiting oxidative stress and apoptotic neuronal death. Dietary supplementation of DHA during pregnancy may thus be beneficial in preventing neonatal brain injury.