Hyperthyroidism decreases thyrotropin-releasing hormone gene expression in the caudal raphe nuclei and the parapyramidal regions in rats.
Hyperthyroidism decreases thyrotropin-releasing hormone gene expression in the caudal raphe nuclei and the parapyramidal regions in rats.
复制标题
甲状腺功能亢进会降低大鼠中缝核尾部和锥体旁区域的促甲状腺素释放激素基因表达。
DOI：
10.1016/s0304-3940(99)00830-7
复制
发表时间：
1999
影响因子：
2.5
通讯作者：
Yang,H
中科院分区：
文献类型：
--
作者：
Yuan,PQ;Yang,H
甲状腺状态改变与自主神经紊乱有关。延髓促甲状腺激素释放激素（TRH）和P物质（SP）调节自主神经活动。应用定量原位杂交组织化学方法研究了甲状腺状态对中缝尾侧核和锥体旁区TRH和SP基因表达的影响。雄性大鼠甲状腺切除（Tx）30天后，血清T4水平下降64%，髓质TRH前体mRNA信号（每个神经元的银颗粒数）显著增加32- 45%。通过每日腹腔注射T4（2 μg/100 g）预防Tx大鼠的这些变化。在假手术/T4（20 μg/100 g，每日）注射大鼠中，T4水平显著增加88%，银颗粒减少38- 40%。髓质SP mRNA信号没有显着改变改变甲状腺状态。这些结果支持甲状腺激素通过负反馈调节髓质TRH基因表达的观点。
Altered thyroid statuses are associated with autonomic disorders. Medullary thyrotropin-releasing hormone (TRH) and substance P (SP) regulate autonomic nervous activity. The influences of thyroid statuses on TRH and SP gene expressions in the caudal raphe nuclei and the parapyramidal regions were studied using quantitative in situ hybridization histochemistry. In male rats thyroidectomized (Tx) for 30 days, the serum T4levels decreased by 64% and the medullary pro-TRH mRNA signals (silver grains per neuron) significantly increased by 32–45%. These changes were prevented by daily i.p. T4(2 μg/100 g) injection in Tx rats. In sham operated/T4(20 μg/100 g, daily) injected rats, T4levels significantly increased by 88% and the silver grains decreased by 38–40%. Medullary SP mRNA signals were not significantly changed by altering thyroid status. These results support the concept that thyroid hormone regulates medullary TRH gene expression by negative feedback.