LIPOSOME-MEDIATED ENZYMATIC-SYNTHESIS OF PHOSPHATIDYLCHOLINE AS AN APPROACH TO SELF-REPLICATING LIPOSOMES
LIPOSOME-MEDIATED ENZYMATIC-SYNTHESIS OF PHOSPHATIDYLCHOLINE AS AN APPROACH TO SELF-REPLICATING LIPOSOMES
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DOI：
10.1021/ja00021a043
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发表时间：
1991-10-09
影响因子：
15
通讯作者：
LUISI, PL
中科院分区：
文献类型：
--
作者：
SCHMIDLI, PK;SCHURTENBERGER, P;LUISI, PL
磷脂酰胆碱合成救助途径的4种酶（sn-甘油-3-磷酸酰基转移酶、1-酰基-sn-甘油-3-磷酸酰基转移酶、磷脂酸磷酸酶和胞二磷酸胆碱磷酸胆碱转移酶）同时结合到大豆磷脂酰胆碱脂质体上。有证据表明，整个酶链在脂质体中被重组，并且合成优先定位于酶以活性结合形式存在的原粒质体膜。不同脂肪酸部分的烷基不同的各种磷脂酰胆碱可以用这种方法合成，并结合到宿主脂质体中，脂质体的平衡大小也相应发生变化。目前的系统也可以被看作是一个自我复制脂质体，并简要讨论了这对化学自创生的影响。
The four enzymes of the salvage pathway for phosphatidylcholine synthesis sn-glycerol-3-phosphate acyltransferase, 1-acyl-sn-glycerol-3-phosphate acyltransferase, phosphatidate phosphatase, and cytidinediphosphocholine phosphocholinetransferase were simulataneously bound to soybean phosphatidylcholine liposomes. Evidence is presented that the entire enzyme chain is reconstituted in the liposomes, and that synthesis is preferentially localized in the proteliposomal membrane where the enzymes are present in an active bound form. Various phosphatidylcholines, differing in the alkyl group of the fatty acid moieties, can be synthesized in this way and incorporated into the host liposomes, with a corresponding change of the equilibrium size of the liposomes. The present system can then also be seen as a self-reproducing liposome, and the implication of this for chemical autopoiesis is briefly discussed.